Anti-Glucokinase antibody - N-terminal (ab37796)

Overview

  • Product nameAnti-Glucokinase antibody - N-terminal
    See all Glucokinase primary antibodies
  • Description
    Rabbit polyclonal to Glucokinase - N-terminal
  • Tested applicationsSuitable for: ELISA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig, Marmoset (common)
  • Immunogen

    Synthetic peptide corresponding to Human Glucokinase (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P35557
    (Peptide available as ab108095)

  • Positive control
    • Human breast carcinoma and hepatocarcinoma tissues; HepG2 and mouse liver tissue cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab37796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/100 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 52 kDa).Can be blocked with Glucokinase peptide (ab108095).
IHC-P 1/50 - 1/100.

Target

  • FunctionCatalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.
  • Tissue specificityIsoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.
  • Involvement in diseaseDefects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) [MIM:125851]; also shortened MODY-2. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    Defects in GCK are the cause of familial hyperinsulinemic hypoglycemia type 3 (HHF3) [MIM:602485]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur.
  • Sequence similaritiesBelongs to the hexokinase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP:D-hexose 6-phosphotransferase antibody
    • FGQTL3 antibody
    • GCK antibody
    • GK antibody
    • GLK antibody
    • Glucokinase antibody
    • Hexokinase D pancreatic isozyme antibody
    • Hexokinase type IV antibody
    • Hexokinase-4 antibody
    • Hexokinase-D antibody
    • HHF3 antibody
    • HK IV antibody
    • HK4 antibody
    • HKIV antibody
    • HXK4_HUMAN antibody
    • HXKP antibody
    • LGLK antibody
    • MODY2 antibody
    see all

Anti-Glucokinase antibody - N-terminal images

  • All lanes : Anti-Glucokinase antibody - N-terminal (ab37796)

    Lane 1 : Hep G2 cell lysate
    Lane 2 : Mouse liver lysate


    Predicted band size : 52 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,37 kDa,50 kDa. We are unsure as to the identity of these extra bands.
  • Formalin fixed, paraffin embedded human breast carcinoma tissue using ab37796, followed by AEC staining.
  • ICC/IF image of ab37796 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37796, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Glucokinase antibody - N-terminal (ab37796)

This product has been referenced in:
  • Li YG  et al. 1-deoxynojirimycin inhibits glucose absorption and accelerates glucose metabolism in streptozotocin-induced diabetic mice. Sci Rep 3:1377 (2013). WB ; Mouse . Read more (PubMed: 23536174) »
  • Li YG  et al. Cooperative anti-diabetic effects of deoxynojirimycin-polysaccharide by inhibiting glucose absorption and modulating glucose metabolism in streptozotocin-induced diabetic mice. PLoS One 8:e65892 (2013). WB ; Mouse . Read more (PubMed: 23755289) »

See all 7 Publications for this product

Product Wall

The current lot we have in stock for ab37796 is GR8879-26. This has a concentration of 2 mg/ml.

Once you receive your order, if you have a different lot please do not hesitate to let me know and we can check the concentration for you.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (HepG2)
Specification HepG2
Treatment +/- Testosterone
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 06 2013

Thank you for contacting us and for reporting the problem you experienced with the antibody ab37796. We will use the data you provided to investigate the source of the problem with this product. I am sorry that the product you received did not perfo...

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Merci de votre intérêt pour les anticorps Abcam. Oui, je recommanderais ab37796 pour le marquage de la glucokinase et ab69508 pour le récepteur insuline beta. Par contre, pour le marquage de PCK1 je recommanderais plutôt un anticorps test...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (pancreatic beta cells)
Loading amount 20 µg
Specification pancreatic beta cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Dr. Sarah Ronnebaum

Verified customer

Submitted Jun 19 2007

Thank you for your patience in this matter. I have received a reply from the originator of ab37796 (IGLK antibody). Glucokinase is actually expressed in HepG2, please follow the links below to a couple of articles that demonstrate this point: h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"