Anti-glucose-6-phosphatase, catalytic subunit antibody (ab96142)


  • Product nameAnti-glucose-6-phosphatase, catalytic subunit antibody
    See all glucose-6-phosphatase, catalytic subunit primary antibodies
  • Description
    Rabbit polyclonal to glucose-6-phosphatase, catalytic subunit
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Cat, Dog, Macaque Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human glucose-6-phosphatase, catalytic subunit.

  • Positive control
    • This antibody gave a positive signal in HEK293 whole cell lysate as well as the following tissue lysates: Human Fetal Liver; Human Fetal Lung; Human Fetal Heart.



Our Abpromise guarantee covers the use of ab96142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 43 kDa (predicted molecular weight: 40 kDa).


  • FunctionHydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production through glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.
  • PathwayCarbohydrate biosynthesis; gluconeogenesis.
  • Involvement in diseaseDefects in G6PC are the cause of glycogen storage disease type 1A (GSD1A) [MIM:232200]. A metabolic disorder characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Patients manifest a wide range of clinical symptoms and biochemical abnormalities, including hypoglycemia, severe hepatomegaly due to excessive accumulation of glycogen, kidney enlargement, growth retardation, lactic acidemia, hyperlipidemia, and hyperuricemia.
  • Sequence similaritiesBelongs to the glucose-6-phosphatase family.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW107337 antibody
    • G-6-Pase antibody
    • G6Pase antibody
    • G6Pase-alpha antibody
    • g6pc antibody
    • G6PC_HUMAN antibody
    • G6PT antibody
    • Glucose-6-phosphatase alpha antibody
    • Glucose-6-phosphatase antibody
    • GSD1 antibody
    • GSD1a antibody
    • MGC163350 antibody
    • MGC93613 antibody
    • RP23-281C18.19 antibody
    see all

Anti-glucose-6-phosphatase, catalytic subunit antibody images

  • All lanes : Anti-glucose-6-phosphatase, catalytic subunit antibody (ab96142) at 1/250 dilution

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)
    Lane 3 : Heart (Human) Whole Cell Lysate - fetal normal tissue
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 13 kDa,15 kDa,21 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-glucose-6-phosphatase, catalytic subunit antibody (ab96142)

ab96142 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (liver)
Loading amount 40 µg
Specification liver
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Oct 22 2012

Expiration date: 21-12-2012

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Thank you for your call today and for letting us know about the trouble with ab83690. It was a pleasure speaking to you.

As we discussed, I'm sending a vial of ab93857 free of charge on the order ***. This is in stock and should arrive tomorr...

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