Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric) (ab107923)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 1 µM
Overview
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Product name
Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric)
See all Glucose 6 phosphate kits -
Detection method
Fluorescent -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 1 µM -
Range
1 µM - 500 µM -
Assay time
0h 20m -
Product overview
Abcam's Glucose-6-Phosphate Assay Kit - High Sensitivity (Fluorometric) (ab107923) is a simple, sensitive and rapid means of quantifying glucose-6-Phosphate (G6P) in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product that converts a nearly colorless probe to an intensely fluorescent product (Ex/Em 535/587 nm). This assay can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 µM in the original sample assuming a dilution of 5X during processing. This Glucose-6-Phosphate Assay Kit - High Sensitivity is more sensitive than our colorimetric assay, Glucose-6-Phosphate Assay Kit (ab83426).
Visit our FAQs page for tips and troubleshooting.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K687 PicoProbe™ Glucose-6-Phosphate Fluorometric Assay Kit. K687-100 is the same size as the 100 test size of ab107923.
Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells which then enters either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Glucose 6 phosphate assay buffer 1 x 25ml Glucose 6 phosphate Enzyme Mix (lyophilized) 1 vial Glucose 6 phosphate Standard (10 µmol; lyophilized) 1 vial Glucose 6 phosphate Substrate Mix (lyophilized) 1 vial Probe (in DMSO) 1 x 0.4ml -
Research areas
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Relevance
Glucose 6 phosphate (G6P) is a key sugar intermediate for glucose to get into cells, and then enter either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. -
Alternative names
- G6P
Images
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Glucose 6 phosphate measured in mouse tissue lysates showing quantity (pmol) per mg protein of tested sample
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Glucose 6 phosphate measured in biological fluids showing quantity (pmol) per microliter of tested sample
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Glucose 6 phosphate standard curves (0 – 500 and 0 – 50 pmol range) generated using ab107923.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab107923 has been referenced in 1 publication.
- Carmean CM et al. Glycogen Repletion in Brown Adipose Tissue upon Refeeding Is Primarily Driven by Phosphorylation-Independent Mechanisms. PLoS One 11:e0156148 (2016). PubMed: 27213961