Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab106810)

Overview

  • Product name
    Anti-Glucose 6 Phosphate Dehydrogenase antibody
    See all Glucose 6 Phosphate Dehydrogenase primary antibodies
  • Description
    Goat polyclonal to Glucose 6 Phosphate Dehydrogenase
  • Specificity
    ab106810 is expected to recognise isoforms NP_000393.4 and NP_001035810.1.
  • Tested applications
    Suitable for: WB, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Sheep, Dog
  • Immunogen

    Synthetic peptide:

    C-STNSDDVRDEKVK

    , corresponding to internal sequence amino acids 308-320 of Human Glucose 6 Phosphate Dehydrogenase (NP_000393.4).

  • Positive control
    • Human Testis, Thyroid and Tonsil lysates; Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab106810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 59 kDa.Can be blocked with Glucose 6 Phosphate Dehydrogenase peptide (ab196304).
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 5 - 10 µg/ml. Increasing amount of recombinant G6PD captured by a rabbit antibody. ab106810 used as reporter antibody.

Target

  • Function
    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • Tissue specificity
    Isoform Long is found in lymphoblasts, granulocytes and sperm.
  • Pathway
    Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • Involvement in disease
    Anemia, non-spherocytic hemolytic, due to G6PD deficiency
  • Sequence similarities
    Belongs to the glucose-6-phosphate dehydrogenase family.
  • Post-translational
    modifications
    Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • G6PD antibody
    • G6PD_HUMAN antibody
    • G6PD1 antibody
    • G6pdx antibody
    • Glucose 6 phosphate 1 dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase, G6PD antibody
    • Glucose-6-phosphate 1-dehydrogenase antibody
    • MET19 antibody
    • POS10 antibody
    • Zwf1p antibody
    see all

Images

  • Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab106810) at 0.03 µg/ml + Human testis lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 59 kDa
  • ab106810, at 2.5 µg/ml, staining Glucose 6 phosphate dehydrogenase in paraffin-embedded Human testis tissue by Immunohistochemistry using AP staining.

References

ab106810 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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