Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab106810)

Overview

  • Product nameAnti-Glucose 6 Phosphate Dehydrogenase antibody
    See all Glucose 6 Phosphate Dehydrogenase primary antibodies
  • Description
    Goat polyclonal to Glucose 6 Phosphate Dehydrogenase
  • Specificityab106810 is expected to recognise isoforms NP_000393.4 and NP_001035810.1.
  • Tested applicationsSuitable for: WB, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Sheep, Dog
  • Immunogen

    Synthetic peptide:

    C-STNSDDVRDEKVK

    , corresponding to internal sequence amino acids 308-320 of Human Glucose 6 Phosphate Dehydrogenase (NP_000393.4).

  • Positive control
    • Human Testis, Thyroid and Tonsil lysates; Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab106810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 59 kDa.Can be blocked with Glucose 6 Phosphate Dehydrogenase peptide (ab196304).
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 5 - 10 µg/ml. Increasing amount of recombinant G6PD captured by a rabbit antibody. ab106810 used as reporter antibody.

Target

  • FunctionCatalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • Tissue specificityIsoform Long is found in lymphoblasts, granulocytes and sperm.
  • PathwayCarbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • Involvement in diseaseAnemia, non-spherocytic hemolytic, due to G6PD deficiency
  • Sequence similaritiesBelongs to the glucose-6-phosphate dehydrogenase family.
  • Post-translational
    modifications
    Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • G6PD antibody
    • G6PD_HUMAN antibody
    • G6PD1 antibody
    • G6pdx antibody
    • Glucose 6 phosphate 1 dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase, G6PD antibody
    • Glucose-6-phosphate 1-dehydrogenase antibody
    • MET19 antibody
    • POS10 antibody
    • Zwf1p antibody
    see all

Anti-Glucose 6 Phosphate Dehydrogenase antibody images

  • Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab106810) at 0.03 µg/ml + Human testis lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 59 kDa
  • ab106810, at 2.5 µg/ml, staining Glucose 6 phosphate dehydrogenase in paraffin-embedded Human testis tissue by Immunohistochemistry using AP staining.

References for Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab106810)

ab106810 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab106810.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"