Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993)

Overview

  • Product name
    Anti-Glucose 6 Phosphate Dehydrogenase antibody
    See all Glucose 6 Phosphate Dehydrogenase primary antibodies
  • Description
    Rabbit polyclonal to Glucose 6 Phosphate Dehydrogenase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide within Human Glucose 6 Phosphate Dehydrogenase aa 50-100. The exact sequence is proprietary.
    Database link: P11413

  • Positive control
    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates;. ICC/IF: HUVEC cells. IHC-Fr: Mouse skeletal muscle tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500.
IP Use at 2-10 µg/mg of lysate.
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • Tissue specificity
    Isoform Long is found in lymphoblasts, granulocytes and sperm.
  • Pathway
    Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • Involvement in disease
    Anemia, non-spherocytic hemolytic, due to G6PD deficiency
  • Sequence similarities
    Belongs to the glucose-6-phosphate dehydrogenase family.
  • Post-translational
    modifications
    Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • G6PD antibody
    • G6PD_HUMAN antibody
    • G6PD1 antibody
    • G6pdx antibody
    • Glucose 6 phosphate 1 dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase, G6PD antibody
    • Glucose-6-phosphate 1-dehydrogenase antibody
    • MET19 antibody
    • POS10 antibody
    • Zwf1p antibody
    see all

Images

  • All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody (ab993) at 1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 59 kDa


    Exposure time: 30 seconds
  • ICC/IF image of ab993 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab993 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Glucose 6 Phosphate Dehydrogenase was immunoprecipitated from NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab993 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab993 at 1 µg/ml.

    Lane 1: ab993 (batch 3) IP in NIH/3T3 whole cell lysate.

    Lane 2: ab993 (batch 4) IP in NIH/3T3 whole cell lysate.

    Lane 3: Control IgG IP in NIH/3T3 whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

  • ab993 staining Glucose 6 phosphate dehydrogenase in Mouse skeletal muscle tissue sections by IHC-Fr (frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS Tween 20) for 12 hours at 4°C. An Alexa Fluor®-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Putker M  et al. Mammalian Circadian Period, But Not Phase and Amplitude, Is Robust Against Redox and Metabolic Perturbations. Antioxid Redox Signal 28:507-520 (2018). Read more (PubMed: 28506121) »
  • Chhabra A  et al. Glucose-6-phosphate dehydrogenase is critical for suppression of cardiac hypertrophy by H2S. Cell Death Discov 4:6 (2018). Read more (PubMed: 29531803) »

See all 17 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry regarding a reference for ab993. The following reference has used this antibody: Jain M et al. Increased myocardial dysfunction after ischemia-reperfusion in mice lacking glucose-6-phosphate dehydrogenase. Circulation 10...

Read More

On the datasheet for ab993, there are two blots shown with a strong band at approx. 65-70 kDa (antibody was used at 1:5000). If the customer has any more questions, please contact us again.

Try this antibody at 1:1000 and adjust accordingly from the results obtained.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up