Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6] (ab91034)

Overview

  • Product nameAnti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6]
    See all Glucose 6 Phosphate Dehydrogenase primary antibodies
  • Description
    Mouse monoclonal [AT2F6] to Glucose 6 Phosphate Dehydrogenase
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human
  • Immunogen

    Recombinant fragment, corresponding to internal sequence amino acids 35-506 of Human Glucose 6 Phosphate Dehydrogenase, purified from E. coli.

  • Positive control
    • MCF7 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab91034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 59 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionCatalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
  • Tissue specificityIsoform Long is found in lymphoblasts, granulocytes and sperm.
  • PathwayCarbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
  • Involvement in diseaseAnemia, non-spherocytic hemolytic, due to G6PD deficiency
  • Sequence similaritiesBelongs to the glucose-6-phosphate dehydrogenase family.
  • Post-translational
    modifications
    Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • G6PD antibody
    • G6PD_HUMAN antibody
    • G6PD1 antibody
    • G6pdx antibody
    • Glucose 6 phosphate 1 dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase antibody
    • Glucose 6 phosphate dehydrogenase, G6PD antibody
    • Glucose-6-phosphate 1-dehydrogenase antibody
    • MET19 antibody
    • POS10 antibody
    • Zwf1p antibody
    see all

Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6] images

  • Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6] (ab91034) at 1/1000 dilution + MCF7 cell lysate at 30 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 59 kDa

References for Anti-Glucose 6 Phosphate Dehydrogenase antibody [AT2F6] (ab91034)

ab91034 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Tissue lysate - whole (Bovine Coronary Artery)
Loading amount 35 µg
Specification Bovine Coronary Artery
Gel Running Conditions Reduced Denaturing (9%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

hirotaka ata

Verified customer

Submitted Apr 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (A7r5)
Loading amount 45 µg
Specification A7r5
Gel Running Conditions Reduced Denaturing (9%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

hirotaka ata

Verified customer

Submitted Apr 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"