Recombinant
RabMAb

Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6291] (ab124738)

Overview

  • Product name
    Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6291]
    See all Glucose 6 Phosphate Dehydrogenase primary antibodies
  • Description
    Rabbit monoclonal [EPR6291] to Glucose 6 Phosphate Dehydrogenase
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Glucose 6 Phosphate Dehydrogenase.

  • Positive control
    • 293T, MOLT4, HUVEC and HeLa cell lysates; Permeablized HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Applications

Our Abpromise guarantee covers the use of ab124738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
    • Tissue specificity
      Isoform Long is found in lymphoblasts, granulocytes and sperm.
    • Pathway
      Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
    • Involvement in disease
      Anemia, non-spherocytic hemolytic, due to G6PD deficiency
    • Sequence similarities
      Belongs to the glucose-6-phosphate dehydrogenase family.
    • Post-translational
      modifications
      Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.
    • Information by UniProt
    • Database links
    • Alternative names
      • G6PD antibody
      • G6PD_HUMAN antibody
      • G6PD1 antibody
      • G6pdx antibody
      • Glucose 6 phosphate 1 dehydrogenase antibody
      • Glucose 6 phosphate dehydrogenase antibody
      • Glucose 6 phosphate dehydrogenase, G6PD antibody
      • Glucose-6-phosphate 1-dehydrogenase antibody
      • MET19 antibody
      • POS10 antibody
      • Zwf1p antibody
      see all

    Images

    • All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6291] (ab124738) at 1/10000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : MOLT4 cell lysate
      Lane 3 : HUVEC cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 59 kDa
    • ab124738 at 1/10 dilution staining Glucose 6 Phosphate Dehydrogenase in permeablized HeLa cells (red) or a rabbit IgG (negative) (green).

    References

    ab124738 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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