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Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529)

Q&A (8)

Overview

  • Product nameGlucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric)
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Sensitivity
    > 0.04 mU/well
  • Assay time
    1h 00m
  • Product overview

    Abcam's Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) is a simple, sensitive and rapid assay detects the activity of Glucose 6 Phosphate Dehydrogenase (G6PDH) in a variety of samples. In the assay, glucose 6 phosphate is oxidized, which leads to the conversion of a nearly colorless probe to an intensely colored product with an absorbance at 450nm.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The G6PDH Assay Kit can detect as low as 0.04mU G6PDH per well.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceGlucose 6 phosphate dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands.
    • Alternative names
        EC=1.1.1.49G6PDG6PD1Glucose 6 phosphate 1 dehydrogenase

    Applications

    Our Abpromise guarantee covers the use of ab102529 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) images

    • Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (ab102529)
      Functional Studies - Glucose 6 Phosphate Dehydrogenase Assay Kit (ab102529)
      Example of Standard curve obtained using ab102529
    • Functional Studies - Glucose 6 Phosphate Dehydrogenase Colorimetric Assay Kit (ab102529)
      Functional Studies - Glucose 6 Phosphate Dehydrogenase Colorimetric Assay Kit (ab102529)
      Example of positive control and pork liver samples test curves obtained using ab102529

    Protocols

    References for Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529)

    ab102529 has not yet been referenced specifically in any publications.

    Publishing research using ab102529 ? Please let us know so that we can cite the reference in this datasheet

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    I am interested in Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529) but I want to know something. I want to perform G6PD activity in plasma. Please tell me it is possible to study plasma with this kit or not. If it is possible please ...

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    Thank you for your enquiry.

    I can confirm that plasma samples can be used with ab102529 Glucose 6 Phosphate Dehydrogenase Assay Kit.

    Here is the plasma preparation protocol:

    Collect whole blood into commercially available ...

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    Does this product Ab 102529 suitable for Human blood sample?

    We have not tried using blood samples with this assay kit. Since blood is coloured, it will interfere with the colour of the reaction and contribute to the final readings. Also, since this is an enzymatic assay, you cannot use a filter to remove the prote...

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    Bonjour,
    Pourriez-vous me donner quelques précisions concernant un kit que vous
    commercialisez:
    kit ab102529 Glucose 6 phosphate Dehydrogenase assay Kit.
    Est-ce que ce kit peut- être utilisé pour le dosage de l'act...

    Read More

    Merci de nous avoir contactés.
    Le kit ab102529 (Glucose 6 Phosphate Dehydrogenase Assay Kit - Colorimetric) peut être utilisé pour des cultures cellulaires.
    Il est recommandé de travailler avec 2 ou 5 millions de cellules homogénéisées avec du PBS...

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    Thanks a lot for your information, which is very useful. We are grateful and completely satisfied with your great support.

    I'm happy to help! Please feel free to contact me with any further questons.


    Thanks for your quick response. That is very helpful.

    We have more questions about the methods used to homogenize the cells.

    + Can the cells be homogenized by using ultrasonication? If yes, is 15-25KHz good enough for doing that...

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    Thank you for your enquiry.

    The cells can be homogenized with sonication on ice. I suggest contacting the supplier of the sonicator to confirm the best settings for cell lysis.

    I would not recommend freeze/thaw but you could also use a...

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    Hello:I want to buy the kit: ab102529 Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) .But I don't know if this kit is suitable for cultured cells?I want to know the answer as soon as possible. Thanks very much.

    For cells with this assay, 2-5 X 10^6 cells can be homogenized with an equivalent volume of ice cold PBS or other buffer (pH 6.5 - 8). Add 1 - 50µl samples into duplicate wells of a 96-well plate and bring volume to 50 µl with Assay Buffer. We...

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    Was it intentional to reference NADH instead of NADPH? The standard curve and the data analysis reference NADH, but I believe it should reference NADPH, as glucose 6 phosphate dehydrogenase generates NADPH.

    Thank you for your patience while I waited to hear back from the lab.

    Without giving up any of the proprietary information, it is a little complex to explain this reaction. Since NADH is one of the intermediates in this the reaction process utili...

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    Was it intentional to reference NADH instead of NADPH? The standard curve and the data analysis reference NADH, but I believe it should reference NADPH, as glucose 6 phosphate dehydrogenase generates NADPH.


    Thank you for contacting Abcam.


    The NADH reference is correct for this kit, which is why we provide the NADH standard.

    I hope this information has been helpful for you.

    Please let me know if you have any other questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"