Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529)

Overview

  • Product nameGlucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric)
    See all Glucose 6 Phosphate Dehydrogenase kits
  • Detection methodColorimetric
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Sensitivity
    > 0.04 mU/well
  • Assay time
    1h 00m
  • Assay durationMultiple steps standard assay
  • Product overview

    Abcam's Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529) is a simple, sensitive and rapid assay detects the activity of Glucose 6 Phosphate Dehydrogenase (G6PDH) in a variety of samples. In the assay, glucose 6 phosphate is oxidized, which leads to the conversion of a nearly colorless probe to an intensely colored product with an absorbance at 450nm.

    The G6PDH Assay Kit can detect as low as 0.04mU G6PDH per well.


    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFunctional Studiesmore details
  • PlatformMicroplate

Properties

  • RelevanceGlucose 6 phosphate dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands.
  • Alternative names
    • EC=1.1.1.49
    • G6PD
    • G6PD1
    • Glucose 6 phosphate 1 dehydrogenase

Applications

Our Abpromise guarantee covers the use of ab102529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) images

  • NADH measured in biological fluids after 2 min and 30 min incubation time showing quantity (nmol) per ml of tested sample.

  • NADH measured in mouse tissue lysates after 2 min and 30 min incubation time showing quantity (nmol) per mg of tested sample.
  • Glucose 6 phosphate dehydrogenase measured in mouse tissue lysates showing quantity (mU) per mg of tested sample.
  • NADH measured in cell lysate after 2 min and 30 min incubation time showing quantity (nmol) per 1 mln of tested cells.

  • G6PD measured in biological fluids showing quantity (mU) per ml of tested sample after 30 min incubation time.

  • Example of positive control and pork liver samples test curves obtained using ab102529
  • Example of Standard curve obtained using ab102529

Protocols

References for Glucose 6 Phosphate Dehydrogenase Assay Kit (Colorimetric) (ab102529)

ab102529 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Methanol lysis holds advantages over both NP-40 and mechanical lysis in that it much quicker, requiring only 2 minutes incubation at room temperature, followed by centrifugation and aspiration of the supernatant. Please find attached data from my experiment which shows that, despite identical NADH standard curves, lysis of human PBMCs in methanol leads to loss of measurable G6PDH activity when compared to NP-40 lysis.
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Verified customer

Submitted Oct 24 2014

This buffer should be fine.

We have not tested this specific combination of components in the concentrations mentioned. So there can always be some unforeseen interference, but theoretically the composition should work with this kit.

Thank you for your enquiry.

I can confirm that plasma samples can be used with ab102529 Glucose 6 Phosphate Dehydrogenase Assay Kit.

Here is the plasma preparation protocol:

Collect whole blood into commercially availab...

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We have not tried using blood samples with this assay kit. Since blood is coloured, it will interfere with the colour of the reaction and contribute to the final readings. Also, since this is an enzymatic assay, you cannot use a filter to remove the pr...

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Merci de nous avoir contactés.
Le kit ab102529 (Glucose 6 Phosphate Dehydrogenase Assay Kit - Colorimetric) peut être utilisé pour des cultures cellulaires.
Il est recommandé de travailler avec 2 ou 5 millions de cellules homogénéisées avec du ...

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I'm happy to help! Please feel free to contact me with any further questons.

Thank you for your enquiry.

The cells can be homogenized with sonication on ice. I suggest contacting the supplier of the sonicator to confirm the best settings for cell lysis.

I would not recommend freeze/thaw but you could also us...

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For cells with this assay, 2-5 X 10^6 cells can be homogenized with an equivalent volume of ice cold PBS or other buffer (pH 6.5 - 8). Add 1 - 50µl samples into duplicate wells of a 96-well plate and bring volume to 50 µl with Assay Buffer....

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Thank you for your patience while I waited to hear back from the lab.

Without giving up any of the proprietary information, it is a little complex to explain this reaction. Since NADH is one of the intermediates in this the reaction process ut...

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Thank you for contacting Abcam.


The NADH reference is correct for this kit, which is why we provide the NADH standard.

I hope this information has been helpful for you.

Please let me know if you have any other questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"