Anti-Glucose 6 phosphate isomerase antibody (ab89596)

Overview

  • Product nameAnti-Glucose 6 phosphate isomerase antibody
    See all Glucose 6 phosphate isomerase primary antibodies
  • Description
    Mouse polyclonal to Glucose 6 phosphate isomerase
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length Human Glucose 6 phosphate isomerase (AAH04982.1)

  • Positive control
    • Human liver tissue lysate. Rat brain tissue lysate. Glucose 6 phosphate isomerase transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab89596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.

Target

  • FunctionBesides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
  • PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
  • Involvement in diseaseDefects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
  • Sequence similaritiesBelongs to the GPI family.
  • Post-translational
    modifications
    Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
    ISGylated.
  • Cellular localizationCytoplasm. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMF antibody
    • Aurocrine motility factor antibody
    • Autocrine motility factor antibody
    • DKFZp686C13233 antibody
    • EC 5.3.1.9 antibody
    • G6PI_HUMAN antibody
    • Glucose phosphate isomerase antibody
    • Glucose-6-phosphate isomerase antibody
    • GNPI antibody
    • GPI antibody
    • Gpi1 antibody
    • Hexose monophosphate isomerase antibody
    • Hexosephosphate isomerase antibody
    • Neuroleukin antibody
    • NLK antibody
    • Oxoisomerase antibody
    • PGI antibody
    • PHI antibody
    • Phosphoglucose isomerase antibody
    • Phosphohexomutase antibody
    • Phosphohexose isomerase antibody
    • Phosphosaccharomutase antibody
    • SA 36 antibody
    • SA-36 antibody
    • SA36 antibody
    • Sperm antigen 36 antibody
    see all

Anti-Glucose 6 phosphate isomerase antibody images

  • Anti-Glucose 6 phosphate isomerase antibody (ab89596) at 1 µg/ml + Human liver tissue lysate at 50 µg

    Predicted band size : 63 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
  • Anti-Glucose 6 phosphate isomerase antibody (ab89596) at 1 µg/ml + Rat brain tissue lysate at 50 µg

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
  • All lanes : Anti-Glucose 6 phosphate isomerase antibody (ab89596) at 1 µg/ml

    Lane 1 : Glucose 6 phosphate isomerase transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 63 kDa
    Observed band size : 63 kDa

References for Anti-Glucose 6 phosphate isomerase antibody (ab89596)

ab89596 has not yet been referenced specifically in any publications.

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