Recombinant
RabMAb

Anti-Glucose 6 phosphate isomerase antibody [EPR11663(B)] (ab167394)

Overview

  • Product name
    Anti-Glucose 6 phosphate isomerase antibody [EPR11663(B)]
    See all Glucose 6 phosphate isomerase primary antibodies
  • Description
    Rabbit monoclonal [EPR11663(B)] to Glucose 6 phosphate isomerase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Glucose 6 phosphate isomerase (UniProt: P06744).

  • Positive control
    • HepG2, HeLa, HT29, and A549 cell lysates; Human prostatic hyperplasia and Human thyroid carcinoma tissues; HepG2 and A549 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 63 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
    • Pathway
      Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
    • Involvement in disease
      Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
    • Sequence similarities
      Belongs to the GPI family.
    • Post-translational
      modifications
      Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
      ISGylated.
    • Cellular localization
      Cytoplasm. Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • AMF antibody
      • Aurocrine motility factor antibody
      • Autocrine motility factor antibody
      • DKFZp686C13233 antibody
      • EC 5.3.1.9 antibody
      • G6PI_HUMAN antibody
      • Glucose phosphate isomerase antibody
      • Glucose-6-phosphate isomerase antibody
      • GNPI antibody
      • GPI antibody
      • Gpi1 antibody
      • Hexose monophosphate isomerase antibody
      • Hexosephosphate isomerase antibody
      • Neuroleukin antibody
      • NLK antibody
      • Oxoisomerase antibody
      • PGI antibody
      • PHI antibody
      • Phosphoglucose isomerase antibody
      • Phosphohexomutase antibody
      • Phosphohexose isomerase antibody
      • Phosphosaccharomutase antibody
      • SA 36 antibody
      • SA-36 antibody
      • SA36 antibody
      • Sperm antigen 36 antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling Glucose 6 phosphate isomerase with purified ab167394 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Control: PBS only

    • ab167394 showing +ve staining in Human normal brain.
    • ab167394 showing +ve staining in Human normal pancreas.
    • ab167394 showing +ve staining in Human ovarian carcinoma.
    • ab167394 showing +ve staining in Human skeletal muscle.
    • All lanes : Anti-Glucose 6 phosphate isomerase antibody [EPR11663(B)] (ab167394) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HT29 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 63 kDa

    • Immunofluorescent analysis of HepG2 cells labeling Glucose 6 phosphate isomerase with ab167394 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue, labeling Glucose 6 phosphate isomerase with ab167394 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling Glucose 6 phosphate isomerase with ab167394 at 1/100 dilution.
    • Flow cytometric analysis of permeabilized A549 cells, labeling Glucose 6 phosphate isomerase with ab167394 at 1/10 dilution.

    References

    ab167394 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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