• Product nameGlucose Assay Kit
    See all Glucose kits
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • Assay typeQuantitative
  • Sensitivity
    1 µM
  • Range
    1 µM - 10000 µM
  • Assay time
    0h 40m
  • Species reactivity
    Reacts with: Mammal
  • Product overview

    Glucose Assay Kit (ab65333) provides direct measurement of glucose in various biological samples (e.g., serum, plasma, body fluid, food, growth medium, etc.). Glucose Enzyme Mix specifically oxidizes glucose to generate a product which reacts with a dye to generate color (λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color and fluorescence is proportionally to the glucose amount. The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. The kit detects 1-10000 µM glucose samples.

    If you have reducing substances in your samples, we recommend using Glucose Detection Kit II (ab102517).

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glucose (C6H12O6; FW: 180.16) is a very important fuel source to generate universal energy molecules ATP. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes.

  • Tested applicationsSuitable for: Functional Studiesmore details


  • RelevanceGlucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.


Our Abpromise guarantee covers the use of ab65333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Glucose Assay Kit images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Glucose measured in cell lysates showing quantity (nmol) per million cells.

    Samples with the concentration of 2x107 cells/mL were used. Samples were diluted 1.5-13.5 fold and measured colorimetrically.

  • Glucose measured in human biological fluids showing quantity (µmol) per mL of tested sample. Samples were diluted 13.5 fold and measured colorimetrically.


References for Glucose Assay Kit (ab65333)

This product has been referenced in:
  • Liu Z  et al. Adiponectin reduces ER stress-induced apoptosis through PPARa transcriptional regulation of ATF2 in mouse adipose. Cell Death Dis 7:e2487 (2016). Mouse . Read more (PubMed: 27882945) »
  • Gao F  et al. Radiation-driven lipid accumulation and dendritic cell dysfunction in cancer. Sci Rep 5:9613 (2015). Read more (PubMed: 25923834) »

See all 11 Publications for this product

Product Wall

The signal contributed by the phenol red in 1ul samples will be insignificant compared to the signal generated by the glucose in your samples. You should include a control consisting of 1ul of culture medium and subtract the absorbance or fluorome...

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Thank you for contacting us. Yes, peroxide is known to interfere with this Glucose Assay Kit. I hope this helps, please let me know if you need any additional information.

This kit is only specific for D-glucose.

I have been in contact with the lab and unfortunately, we have not tested whether the addition of acids such as H2SO4 or PFA will interfere with the assay.

They have suggested that in this situation, perhaps it is best if you deproteinise you...

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We have not tested glucose in purified polysaccharide samples. The first step of the reaction is specific for glucose, so we expect this to work. It's possible that there will be some background, but this can be corrected for by subtracting the backgro...

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The main difference between these two glucose assays is that in ab65333 the probe reacts with hydrogen peroxide and gets oxidized to produce colored compound detectable at 570nm or at Ex/Em 535/587nm. In ab102517, the probe reacts with NADH (produced d...

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I suggest exchanging the medium before collecting samples. That is, culture the cells in medium with a glucose/energy source and then exchange the medium with glucose-free medium such as Krebs-Ringer-Phosphate-HEPES (KRPH) buffer for example, and then ...

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Thank you for your enquiry.

Answer 1: Yes, the minimal serum volume for this assay is 0.5 µl. The lower detection limit of this assay is 1 µM. That does not mean that you have to start with a particular conc of the sample. Mo...

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This kit would be used to measure glucose in the cell culture supernatant. The number of cells in culture would have an effect on the glucose consumption, but the amount of time the cells are cultured and the specific cell line you are testing for will...

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Thank you for your enquiry which has been forwarded to the scientific support team.

I can confirm I would suggest Glucose Detection Kit ab102517or ab65333 Glucose Assay Kitswill be suitable for detection andquantification of glucose in the c...

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