Anti-Glucose Dehydrogenase antibody (Biotin) (ab34539)

Overview

  • Product name
    Anti-Glucose Dehydrogenase antibody (Biotin)
    See all Glucose Dehydrogenase primary antibodies
  • Description
    Goat polyclonal to Glucose Dehydrogenase (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: WB, ELISA, ICC/IF, Immunomicroscopymore details
  • Species reactivity
    Reacts with Bacillus species.
  • Immunogen

    Full length protein [Bacillus species]

  • General notes

    Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.

Properties

Applications

Our Abpromise guarantee covers the use of ab34539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
Immunomicroscopy
  • Application notes
    ELISA: 1/4000 - 1/20000. This product has been assayed against 1.0µg of Glucose Dehydrogenase in a standard capture ELISA using Peroxidase conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 28 kDa.
    Suitable for antibody based assays using Streptavidin or Avidin conjugates requiring lot-to-lot consistency.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Glucose Dehydrogenase catalyses the oxidation of D glucose without prior phosphorylation to D beta gluconolactone using NAD or NADP as a coenzyme. The enzyme is a tetrameric protein, each of the 4 identical subunits containing 262 amino acid residues. This family is a subset of a more general family of short chain dehydrogenases and reductases.
    • Alternative names
      • gdh antibody
      • gdhB antibody
      • Glucose 1 dehydrogenase 1 antibody
      • Glucose 1 dehydrogenase antibody
      • Glucose 1-dehydrogenase B antibody
      see all

    References

    ab34539 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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