Glucose Dehydrogenase Assay Kit (Colorimetric) (ab102532)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell culture media, Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.01 mU/well
Overview
-
Product name
Glucose Dehydrogenase Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media -
Assay type
Enzyme activity -
Sensitivity
> 0.01 mU/well -
Assay time
2h 00m -
Product overview
Abcam's Glucose Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of the GDH in a variety of samples. The GDH present in sample will recognize D-glucose as a specific substrate leading to a proportional color development. The activity of GDH can be easily quantified colorimetrically (λ = 450 nm).
Visit our FAQs page for tips and troubleshooting. -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K786 Glucose Dehydrogenase Activity Colorimetric Assay Kit. K786-100 is the same size as the 100 test size of ab102532.
This assay detects GDH activity as low as 0.01 mU.
Developer (Lyophilized) is light sensitive and should be protected from light during storage and preparation.
-
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Developer (Lyophilized) 1 vial GDH Assay Buffer 1 x 25ml GDH Positive Control (Lyophilized) 1 vial Glucose 1 x 1ml NADH Standard (Lyophilized) 1 vial -
Research areas
-
Relevance
Glucose dehydrogenase (NAD+) (EC 1.1.1.118) is an enzyme that catalyzes the chemical reaction: D-glucose + NAD+ = D-glucono-1,5-lactone + NADH + H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. -
Alternative names
- GDH
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab102532 has been referenced in 1 publication.
- Yalçin A et al. PKR inhibitors suppress endoplasmic reticulum stress and subdue glucolipotoxicity-mediated impairment of insulin secretion in pancreatic beta cells. Turk J Biol 44:93-102 (2020). PubMed: 32256145