Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
Assay typeEnzyme activity
> 0.01 mU/well
Abcam's Glucose Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of the GDH in a variety of samples. The GDH present in sample will recognize D-glucose as a specific substrate leading to a proportional color development. The activity of GDH can be easily quantified colorimetrically (? = 450 nm). Visit our FAQs page for tips and troubleshooting.
This assay detects GDH activity as low as 0.01 mU.
RelevanceGlucose dehydrogenase (NAD+) (EC 220.127.116.11) is an enzyme that catalyzes the chemical reaction: D-glucose + NAD+ = D-glucono-1,5-lactone + NADH + H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.