Overview

  • Product nameGlucose Detection Kit II
    See all Glucose kits
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.02 mM
  • Range
    0.02 mM - 10 mM
  • Assay time
    0h 30m
  • Product overview

    Glucose Detection Kit II (ab102517) provides direct measurement of glucose in various biological samples, and it is particularly suitable for serum and urine samples since it is unaffected by reducing substances, which can interfere with detection in oxidase-based kits. In this assay, glucose is specifically oxidized to generate a product which reacts with a dye to generate color (λ = 450 nm) whose intensity is proportional to glucose concentration. The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. The kit can detect glucose concentrations in the range of 20µM-10mM.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glucose is an important fuel source to generate the universal energy molecule ATP. Serum glucose level is a key diagnostic parameter for many metabolic disorders.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • RelevanceGlucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.

Applications

Our Abpromise guarantee covers the use of ab102517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Glucose Detection Kit II images

  • Glucose measured in biological fluids. Human samples diluted 20-80 fold. Mouse samples diluted 1-27 fold.

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Standard curve for glucose run using the kit protocol

Protocols

References for Glucose Detection Kit II (ab102517)

ab102517 has not yet been referenced specifically in any publications.

Product Wall

The main difference between these two glucose assays is that in ab65333 the probe reacts with hydrogen peroxide and gets oxidized to produce colored compound detectable at 570nm or at Ex/Em 535/587nm. In ab102517, the probe reacts with NADH (produced d...

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Thank you for your enquiry which has been forwarded to the scientific support team.

I can confirm I would suggest Glucose Detection Kit ab102517or ab65333 Glucose Assay Kitswill be suitable for detection andquantification of glucose in the c...

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Thakn you for your enquiry.
I am pleased to provide the further information below regarding the difference between these kits:


Measurement:

ab65333
Absorbance 570 nm or Fluorescence Ex/Em 535/587 nm

ab102...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"