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Glucose Detection Kit II (ab102517) provides direct measurement of glucose in various biological samples, and it is particularly suitable for serum and urine samples since it is unaffected by reducing substances, which can interfere with detection in oxidase-based kits. In this assay, glucose is specifically oxidized to generate a product which reacts with a dye to generate color (λ = 450 nm) whose intensity is proportional to glucose concentration. The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose level during fermentation and glucose feeding in protein expression processes. The kit can detect glucose concentrations in the range of 20µM-10mM.
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Glucose is an important fuel source to generate the universal energy molecule ATP. Serum glucose level is a key diagnostic parameter for many metabolic disorders.
|Glucose Assay Buffer||WM||1 x 25ml|
|Glucose Enzyme Mix||Green||1 unit|
|Glucose Standard||Yellow||1 x 100µl|
|Glucose Substrate Mix||Red||1 unit|
Our Abpromise guarantee covers the use of ab102517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Glucose measured in biological fluids. Human samples diluted 20-80 fold. Mouse samples diluted 1-27 fold.
ab102517 has not yet been referenced specifically in any publications.