Anti-Glucose Transporter 5 GLUT5 antibody (ab41533)

Overview

  • Product nameAnti-Glucose Transporter 5 GLUT5 antibody
    See all Glucose Transporter 5 GLUT5 primary antibodies
  • Description
    Rabbit polyclonal to Glucose Transporter 5 GLUT5
  • Tested applicationsSuitable for: Dot blot, ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from the C terminus of (Human) Glucose Transporter 5 GLUT5. The peptide was post-translationaly modified to achieve maximum antigenicity.

  • Positive control
    • The antibody labels glucose transporter (Glut 5) species from rat jejunal enterocyte basolateral membranes, PC12 cells and rat kidney cortical membranes.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab41533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/4000.
ELISA 1/4000.
WB 1/1000. Predicted molecular weight: 55 kDa.Can be blocked with Glucose Transporter 5 GLUT5 peptide (ab171722).
IP Use at an assay dependent dilution.

Target

  • FunctionCytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter.
  • Tissue specificityExpressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue.
  • Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Facilitated glucose transporter member 5 antibody
    • Fructose transporter antibody
    • Glucose transporte, kidney antibody
    • glucose transporter like protein 5 antibody
    • Glucose transporter type 5 antibody
    • Glucose transporter type 5 small intestine antibody
    • GLUT 5 antibody
    • GLUT-5 antibody
    • GLUT5 antibody
    • GTR5_HUMAN antibody
    • SLC 2A5 antibody
    • SLC2A5 antibody
    • small intestine antibody
    • solute carrier family 2 (facilitated glucose/fructose transporter), member 5 antibody
    • Solute carrier family 2 antibody
    see all

Anti-Glucose Transporter 5 GLUT5 antibody images

  • Anti-Glucose Transporter 5 GLUT5 antibody (ab41533) at 1 µg/ml + Human small intestine tissue lysate - total protein (ab29276) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 25 kDa,71 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Glucose Transporter 5 GLUT5 antibody (ab41533)

This product has been referenced in:
  • Gonzalo-Gobernado R  et al. Neuroprotective activity of peripherally administered liver growth factor in a rat model of Parkinson's disease. PLoS One 8:e67771 (2013). WB . Read more (PubMed: 23861803) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Kidney from 3 week old mouse)
Specification Kidney from 3 week old mouse
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TBE
Permeabilization Yes - 0.1% Triton X100
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
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Verified customer

Submitted May 14 2013

Vielen Dank für Ihre Antwort.
Die Referenznummer Ihrer Gutschrift ist xxxxxx.
Es tut mir leid, dass der Antikörper nicht so funktioniert hat, wie auf dem Datenblatt angegeben. Ich habe unsere Finanzabteilung beauftragt, eine Gutschrift für Si...

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Vielen Dank für Ihre Antwort und für diese weiteren Informationen.
Es tut mir Leid dass Sie diese Probleme mit unserem Antikörper hatten. Ich weiß, dass Sie viel Zeit für diese Experimente verwendet haben und möchte Ihnen daher ein Ersatzröhrchen ...

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Vielen Dank für Ihre Anfrage und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.
Es tut mir leid, dass Sie Probleme mit unserem Anti-Glucose Transporter 5 GLUT5 antibody (ab41533) Antikörper hatten.
Ich möchte Ih...

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Vielen Dank für Ihre Email.
Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.
Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogens erhalten wir alle nötigen Inform...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"