The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 51 kDa.
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Glucose transporter 8 is an insulin-regulated facilitative glucose transporter. It binds cytochalasin B in a glucose-inhibitable manner. It appears to be a dual-specific sugar transporter as it can be inhibited by fructose. It is highly expressed in testis, where it is down-regulated by estrogen, but not in testicular carcinoma. Lower amounts are present in most other tissues.
Cell Membrane; multi-pass membrane protein. Principally intracellular. May move between intracellular vesicles and the plasma membrane