The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 51 kDa).
There are publications supporting the capability of GLUT8 to migrate at 35 kDa. See PMID 10671487 and 10860996. Thus, full length GLUT8 may migrate between 35-51 kDa.
Is unsuitable for ICC/IF,IHC-P or IP.
Glucose transporter 8 is an insulin-regulated facilitative glucose transporter. It binds cytochalasin B in a glucose-inhibitable manner. It appears to be a dual-specific sugar transporter as it can be inhibited by fructose. It is highly expressed in testis, where it is down-regulated by estrogen, but not in testicular carcinoma. Lower amounts are present in most other tissues.
Cell Membrane; multi-pass membrane protein. Principally intracellular. May move between intracellular vesicles and the plasma membrane