Recombinant Anti-Glucose Transporter 8 antibody [EPR9477] (ab169779)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9477] to Glucose Transporter 8
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Glucose Transporter 8 antibody [EPR9477]
See all Glucose Transporter 8 primary antibodies -
Description
Rabbit monoclonal [EPR9477] to Glucose Transporter 8 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human cerebellum, skeletal muscle and fetal heart tissue lysates; Mouse and Rat brain tissue lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9477 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 51 kDa).
There are publications supporting the capability of GLUT8 to migrate at 35 kDa. See PMID 10671487 and 10860996. Thus, full length GLUT8 may migrate between 35-51 kDa. |
Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 51 kDa). There are publications supporting the capability of GLUT8 to migrate at 35 kDa. See PMID 10671487 and 10860996. Thus, full length GLUT8 may migrate between 35-51 kDa. |
Target
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Relevance
Glucose transporter 8 is an insulin-regulated facilitative glucose transporter. It binds cytochalasin B in a glucose-inhibitable manner. It appears to be a dual-specific sugar transporter as it can be inhibited by fructose. It is highly expressed in testis, where it is down-regulated by estrogen, but not in testicular carcinoma. Lower amounts are present in most other tissues. -
Cellular localization
Cell Membrane; multi-pass membrane protein. Principally intracellular. May move between intracellular vesicles and the plasma membrane -
Database links
- Entrez Gene: 29988 Human
- Entrez Gene: 56017 Mouse
- Entrez Gene: 85256 Rat
- Omim: 605245 Human
- SwissProt: Q9NY64 Human
- SwissProt: Q9JIF3 Mouse
- SwissProt: Q9JJZ1 Rat
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Alternative names
- Glucose transporter type 8 antibody
- Glucose transporter type X1 antibody
- GLUT8 antibody
see all
Images
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All lanes : Anti-Glucose Transporter 8 antibody [EPR9477] (ab169779) at 1/1000 dilution (Purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration: 5% NFDM/TBST
The observed bands are consistent with what are described in PMID:10860996
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Anti-Glucose Transporter 8 antibody [EPR9477] (ab169779) at 1/1000 dilution (Purified) + Human skeletal muscle lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration: 5% NFDM/TBST
The observed bands are consistent with what are described in PMID:10860996 -
All lanes : Anti-Glucose Transporter 8 antibody [EPR9477] (ab169779) at 1/1000 dilution (unpurified)
Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 51 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab169779 has been referenced in 4 publications.
- Janzen C et al. Humanin (HN) and glucose transporter 8 (GLUT8) in pregnancies complicated by intrauterine growth restriction. PLoS One 13:e0193583 (2018). PubMed: 29590129
- Shin BC et al. Neural Deletion of Glucose Transporter Isoform 3 Creates Distinct Postnatal and Adult Neurobehavioral Phenotypes. J Neurosci 38:9579-9599 (2018). PubMed: 30232223
- Papageorgiou I et al. Impaired stimulation of glucose transport in cardiac myocytes exposed to very low-density lipoproteins. Nutr Metab Cardiovasc Dis 26:614-622 (2016). PubMed: 27052924
- Zhang X et al. Reactive oxygen species-induced TXNIP drives fructose-mediated hepatic inflammation and lipid accumulation through NLRP3 inflammasome activation. Antioxid Redox Signal 22:848-70 (2015). PubMed: 25602171