Overview

  • Product nameAnti-Glucose Transporter GLUT1 antibody
    See all Glucose Transporter GLUT1 primary antibodies
  • Description
    Rabbit polyclonal to Glucose Transporter GLUT1
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, ICC/IF, ELISA, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human
    Predicted to work with: Horse, Chicken, Cat, Pig, Chimpanzee, Monkey, Baboon
  • Immunogen

    Synthetic peptide corresponding to Rat Glucose Transporter GLUT1 aa 481-492 (C terminal) (Cysteine residue).
    Sequence:

    [C]GLFHPLGADSQV


    Database link: P11167
    (Peptide available as ab187025)

  • Positive control
    • Rat hippocampal plasma membranes. The antibody labels glucose transporter (Glut 1) species from rat brain, human RBC, SHSY5Y human neuroblastoma cells and rat Sertoli cells. Glut 1 transporter distributions is ubiquitous, and is found in red blood cells, microvasculature of brain etc.

Properties

Applications

Our Abpromise guarantee covers the use of ab14683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/200.
ELISA 1/12000.
WB 1/2500. Detects a band of approximately 50 kDa (predicted molecular weight: 55 kDa). We suggest that samples should not be heated or frozen.
IP Use at an assay dependent concentration.

Target

  • FunctionFacilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
  • Tissue specificityExpressed at variable levels in many human tissues.
  • Involvement in diseaseDefects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
    Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
  • Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
    • CSE antibody
    • DYT17 antibody
    • DYT18 antibody
    • DYT9 antibody
    • EIG12 antibody
    • erythrocyte/brain antibody
    • Erythrocyte/hepatoma glucose transporter antibody
    • facilitated glucose transporter member 1 antibody
    • Glucose transporter 1 antibody
    • Glucose transporter type 1 antibody
    • Glucose transporter type 1, erythrocyte/brain antibody
    • GLUT antibody
    • GLUT-1 antibody
    • GLUT1 antibody
    • GLUT1DS antibody
    • GLUTB antibody
    • GT1 antibody
    • GTG1 antibody
    • Gtg3 antibody
    • GTR1_HUMAN antibody
    • HepG2 glucose transporter antibody
    • HTLVR antibody
    • Human T cell leukemia virus (I and II) receptor antibody
    • MGC141895 antibody
    • MGC141896 antibody
    • PED antibody
    • RATGTG1 antibody
    • Receptor for HTLV 1 and HTLV 2 antibody
    • SLC2A1 antibody
    • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 1 antibody
    see all

Anti-Glucose Transporter GLUT1 antibody images

  • All lanes : Anti-Glucose Transporter GLUT1 antibody (ab14683) at 1/2500 dilution

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    This antibody labels Glut 1 protein as a smear due to large amount of glycosylation on Glut 1 transporter. We observed that boiling and freezing the Glut 1 samples resulted in aggregated form of the protein and gives a smear form high 100kDa range to 45kDa with significant reduction in Glut 1 (44-47kDa) band. We suggest that samples should not be heated or frozen. Following extraction in lysis buffer, the proteins are then dissolved in SDS-PAGE sample buffer 2X and reduced in presence of 2.5-5% BME. All samples at this time will be heated 70-80°C for 3 minutes and aliquot and stored frozen till it is time for analysis. Just before analyses the samples much be thawed at room temp or at 37°C to dissolve any precipitated SDS in the sample. The remaining sample must not be frozen again, this freeze and next thaw cycle will make aggregate in some samples.
  • Human normal placenta. Staining is observed at the cell surface. Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX.Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ICC/IF image of ab14683 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14683, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of mammary glands taken from wild-type or Erbb4-/- lactating mice, staining Glucose Transporter GLUT1 with ab14683.

References for Anti-Glucose Transporter GLUT1 antibody (ab14683)

This product has been referenced in:
  • Jiang Y  et al. Hypoxia Potentiates the Radiation-Sensitizing Effect of Olaparib in Human Non-Small Cell Lung Cancer Xenografts by Contextual Synthetic Lethality. Int J Radiat Oncol Biol Phys 95:772-81 (2016). IHC-P . Read more (PubMed: 27020103) »
  • Iwasaki K  et al. Role of hypoxia-inducible factor-1a, carbonic anhydrase-IX, glucose transporter-1 and vascular endothelial growth factor associated with lymph node metastasis and recurrence in patients with locally advanced cervical cancer. Oncol Lett 10:1970-1978 (2015). IHC-P ; Human . Read more (PubMed: 26622782) »

See all 19 Publications for this product

Product Wall

Thank you for your email.

All these antibodies are excellent in detecting GLUT1 by western blot and are fully guaranteed. I can however suggest using ab15309; http://www.abcam.com/Glucose-Transporter-GLUT1-antibody-ab15309.html
which react...

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Thank you for contacting us.

Could you please explain what type of actualized data you would be interested in to see?

All three antibodies has been tested in WB and gave satisfactory results which is why WB application is guaranteed. ...

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Thank you for contacting us.
We always recommend our customers trying different procedure to get excellent results with antibodies sin IHC-P. It is a tricky applications due to antigen retrieval method involved.
Though citrate buffer pH 6.0 w...

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I am so sorry for the long delay in our answer.

Indeed, the laboratory of one of the antibodies against GLUT1 was searching for an image, as they had performed the Western blot on erythrocyte ghost membranes about 10 years ago. However even a...

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I am very pleased to hear you would like to accept our offer and test ab14683 on chicken samples. This code will give you one free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for chicken and include this...

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Thank you for contacting Abcam.
For ab14683, we had not actually tested this antibody on chicken tissue, but based on sequence homology we thought there was a chance it would, hence the reason we said that it was predicted to work with this specie...

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Thank you for your phone call yesterday and for your patience while I have been in touch with the lab about ab14683. Based on the immunogen sequence, the antibody is predicted to react with chicken samples. However, since this has not been tested ye...

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I have received the following recommendations from the laboratory: Extract cellular and membrane proteins in lysis buffer. The proteins were then dissolved in SDS-PAGE sample buffer 2X and reduced the in presence of 2.5-5% BME. All samples at this...

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I have received confirmation from the laboratory that tested ab14683 that this antibody labels Glut 1 protein as a smear due to large amount of glycosylation on Glut 1 transporter. We have also seen that boiling and freezing the Glut 1 samples resulted...

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Thank you for your enquiry. Unfortunately, we are not specialists in this area and we do not want to recommend a product that is unsuitable for your customer's needs. We suggest that your customer refers to the latest literature in this field and a...

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"