Anti-Glucose Transporter GLUT1 antibody (ab15309)

Overview

  • Product name
    Anti-Glucose Transporter GLUT1 antibody
    See all Glucose Transporter GLUT1 primary antibodies
  • Description
    Rabbit polyclonal to Glucose Transporter GLUT1
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive).

  • Positive control
    • HepG2 cells, esophagous and breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab15309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 25269858

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. See Abreview.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 55 kDa.

The band may look broad like that for most membrane glycoproteins. A reviewer of another antibody against Glut1, ab652, suggests "do not boil sample before loading as this causes smearing of GLUT-1 bands".

IHC-P 1/200.

Target

  • Function
    Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
  • Tissue specificity
    Expressed at variable levels in many human tissues.
  • Involvement in disease
    Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
    Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
  • Sequence similarities
    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
    • CSE antibody
    • DYT17 antibody
    • DYT18 antibody
    • DYT9 antibody
    • EIG12 antibody
    • erythrocyte/brain antibody
    • Erythrocyte/hepatoma glucose transporter antibody
    • facilitated glucose transporter member 1 antibody
    • Glucose transporter 1 antibody
    • Glucose transporter type 1 antibody
    • Glucose transporter type 1, erythrocyte/brain antibody
    • GLUT antibody
    • GLUT-1 antibody
    • GLUT1 antibody
    • GLUT1DS antibody
    • GLUTB antibody
    • GT1 antibody
    • GTG1 antibody
    • Gtg3 antibody
    • GTR1_HUMAN antibody
    • HepG2 glucose transporter antibody
    • HTLVR antibody
    • Human T cell leukemia virus (I and II) receptor antibody
    • MGC141895 antibody
    • MGC141896 antibody
    • PED antibody
    • RATGTG1 antibody
    • Receptor for HTLV 1 and HTLV 2 antibody
    • SLC2A1 antibody
    • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 1 antibody
    see all

Images

  • ab15309 staining Glucose Transporter GLUT1 (green) in Human red blood cells tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/500 in PBS-T + 1% PBS) for 12 hours. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Red - autofluorescence of erythrocytes.

    See Abreview

  • ab15309 staining Glucose Transporter GLUT1 in human esophagous by Immunohistochemistry (FFPE-sections).
  • ab15309 at a 1/100 dilution staining rat cells (neural stem cells from adult subventricular zone) by Immunocytochemistry/Immunofluorescence. The cells were incubated with the antibody for 18 hours and then bound antibody was detected using a Cy3 conjugated Goat anti-rabbit IgG (H + L).

    This image is courtesy of an Abreview submitted by Martin Maurer.

    See Abreview

  • ab15309 at 1/300 dilution staining GLUT1 in human fetal heart by immunohistochemistry (frozen sections). Sections were paraformaldehyde fixed, permeabilized in saponin 0.1% in PBS prior to blocking in 10% serum for 45 minutes at 37°C and then incubated with ab15309 for 1 hour at 37°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/600, was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab15309 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15309, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Pahk K  et al. Visualization of Synthetic Vascular Smooth Muscle Cells in Atherosclerotic Carotid Rat Arteries by F-18 FDG PET. Sci Rep 7:6989 (2017). Read more (PubMed: 28765576) »
  • Lacroix M  et al. E4F1 controls a transcriptional program essential for pyruvate dehydrogenase activity. Proc Natl Acad Sci U S A 113:10998-1003 (2016). IHC-P . Read more (PubMed: 27621446) »

See all 39 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (beta cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH6
Permeabilization
No
Specification
beta cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 07 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (canine sarcoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
canine sarcoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
10% NBF
Username

Abcam user community

Verified customer

Submitted Aug 24 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (melanoma cell line xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
melanoma cell line xenograft
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
10% NBF
Username

Abcam user community

Verified customer

Submitted Aug 24 2016

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (H1299 Lung cancer cells)
Specification
H1299 Lung cancer cells
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

Submitted Feb 17 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (human Jurkat (1), mouse 3T3 (2), mouse kidney (3))
Specification
human Jurkat (1), mouse 3T3 (2), mouse kidney (3)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Sep 30 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric buffer, pH6.0
Sample
Human Tissue sections (human red blood cells)
Specification
human red blood cells
Permeabilization
Yes - Tween-20
Fixative
Formaldehyde
Username

Mr. Heiko Locher

Verified customer

Submitted Nov 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma primary cells)
Loading amount
25 µg
Specification
Glioblastoma primary cells
Gel Running Conditions
Reduced Denaturing (12.5%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 07 2013

Thank you for contacting us.

The image which you are referring to was provided to us in an Abreview by a customer. As such do not have all of the specifics of the experiment and cannot know which factors may have lead to the bands showing ...

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Thank you for contacting us.

The two images for WB on the datasheet are under the Abreviews section, so they are results that customers have provided us and not references. One of these reviews does report the band at the "expcetd"...

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Thank you for taking the time to complete our questionnaire. The details you have kindly provided will provide us with vital information for our monitoring of product quality

I appreciate the time you have spent in the laboratory and underst...

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