Anti-Glucose Transporter GLUT1 antibody (ab652)

Overview

  • Product nameAnti-Glucose Transporter GLUT1 antibody
    See all Glucose Transporter GLUT1 primary antibodies
  • Description
    Rabbit polyclonal to Glucose Transporter GLUT1
  • SpecificityGLUT1.
  • Tested applicationsSuitable for: ICC/IF, IHC-FoFr, ICC, Immunomicroscopy, IP, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 478-492 of Human Glucose Transporter GLUT1 (Peptide available as ab115830.)

  • Positive control
    • renal cell carcinoma lysate (see Abreview) human erythocyte membranes

Properties

Applications

Our Abpromise guarantee covers the use of ab652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
ICC 1/1000.
Immunomicroscopy Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa.Can be blocked with Human Glucose Transporter GLUT1 peptide (ab115830). We would not recommend boiling due to the possible irreversible aggregation of glycose transporters. If samples are boiled it can prevent some of the protein from entering the gel or produce multimers which are often mistaken for background. Samples should be solubilized in standard SDS Laemmli buffer and maintained at room temperature before loading.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionFacilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
  • Tissue specificityExpressed at variable levels in many human tissues.
  • Involvement in diseaseDefects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
    Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
  • Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
    • CSE antibody
    • DYT17 antibody
    • DYT18 antibody
    • DYT9 antibody
    • EIG12 antibody
    • erythrocyte/brain antibody
    • Erythrocyte/hepatoma glucose transporter antibody
    • facilitated glucose transporter member 1 antibody
    • Glucose transporter 1 antibody
    • Glucose transporter type 1 antibody
    • Glucose transporter type 1, erythrocyte/brain antibody
    • GLUT antibody
    • GLUT-1 antibody
    • GLUT1 antibody
    • GLUT1DS antibody
    • GLUTB antibody
    • GT1 antibody
    • GTG1 antibody
    • Gtg3 antibody
    • GTR1_HUMAN antibody
    • HepG2 glucose transporter antibody
    • HTLVR antibody
    • Human T cell leukemia virus (I and II) receptor antibody
    • MGC141895 antibody
    • MGC141896 antibody
    • PED antibody
    • RATGTG1 antibody
    • Receptor for HTLV 1 and HTLV 2 antibody
    • SLC2A1 antibody
    • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 1 antibody
    see all

Anti-Glucose Transporter GLUT1 antibody images

  • ICC/IF image of ab652 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab652, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab652 at 1/250 staining human renal carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed before incubation with the primary antibody for 45 minutes. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • All lanes : Anti-Glucose Transporter GLUT1 antibody (ab652) at 1/1000 dilution

    Lane 1 : 2 week old Mouse brain tissue lysate
    Lane 2 : 2 week old Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat HRP-conjugate anti-rabbit at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab652 staining Glucose Transporter GLUT1 in mouse liver tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 before blocking with 2% BSA was performed for 30 minutes at 200C. The sample was incubated with primary antibody (1/200) for 9 hours at 40C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at dilution at 1/200. DAPI was used to stain the cell nuclei (blue).

    See Abreview

  • ab652 staining Glucose Transporter GLUT1 in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/250 for 9 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).

    See Abreview

References for Anti-Glucose Transporter GLUT1 antibody (ab652)

This product has been referenced in:
  • Ambrose LJ  et al. Investigating Mitochondrial Metabolism in Contracting HL-1 Cardiomyocytes Following Hypoxia and Pharmacological HIF Activation Identifies HIF-Dependent and Independent Mechanisms of Regulation. J Cardiovasc Pharmacol Ther N/A:N/A (2014). WB ; Mouse . Read more (PubMed: 24607765) »
  • Nopparat J  et al. d-Catenin, a Wnt/ß-catenin modulator, reveals inducible mutagenesis promoting cancer cell survival adaptation and metabolic reprogramming. Oncogene 0:N/A (2014). Read more (PubMed: 24727894) »

See all 48 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse brain, liver, retina, muscle)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 25 µg
Specification Mouse brain, liver, retina, muscle
Blocking step Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Jul 20 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (necrotic canine sarcoma)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization No
Specification necrotic canine sarcoma
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative Formaldehyde
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Submitted Dec 18 2015

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (MCF7)
Specification MCF7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 25 2015

The antibody ab652 binds to a cytoplasmic region of GLUT1.

This antibody has not been purified and therefore its concentration has not been determined. It is a whole antiserum and so we estimate the concentration to be within the range of 1-10 mg/ml.
As for the negative control, I would suggest using an is...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum (goat) 1/50 diluted in PBS BSA5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: buffer citrate
Sample Human Tissue sections (orbital fat)
Specification orbital fat
Permeabilization No
Fixative Formaldehyde
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Submitted Aug 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Sample Rat Cell (thyrocytes (FRTL-5))
Specification thyrocytes (FRTL-5)
Permeabilization Yes - Saponin 0.1%
Fixative Paraformaldehyde 4%
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Submitted Aug 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (cells were lysated in Laemmli buffer, gel 10 % acrylamide, nitrocellulose membrane)
Sample Rat Cell lysate - whole cell (thyrocytes (FRTL-5))
Specification thyrocytes (FRTL-5)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted Aug 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01 M Tris HCL, pH 10
Sample Mouse Tissue sections (mouse brain tissue)
Specification mouse brain tissue
Permeabilization Yes - 0.3% Triton X-100 10 min
Fixative Paraformaldehyde
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Submitted Jun 11 2013

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (2-wk-old mouse brain tissue)
Specification 2-wk-old mouse brain tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 10 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"