Anti-Glucose Transporter GLUT1 antibody (ab652)

Overview

  • Product name
    Anti-Glucose Transporter GLUT1 antibody
    See all Glucose Transporter GLUT1 primary antibodies
  • Description
    Rabbit polyclonal to Glucose Transporter GLUT1
  • Specificity
    GLUT1.
  • Tested applications
    Suitable for: ICC/IF, IHC-FoFr, ICC, Immunomicroscopy, IP, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 478-492 of Human Glucose Transporter GLUT1 (Peptide available as ab115830.)

  • Positive control
    • renal cell carcinoma lysate (see Abreview) human erythocyte membranes

Properties

Applications

Our Abpromise guarantee covers the use of ab652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
ICC 1/1000.
Immunomicroscopy Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa. We would not recommend boiling due to the possible irreversible aggregation of glycose transporters. If samples are boiled it can prevent some of the protein from entering the gel or produce multimers which are often mistaken for background. Samples should be solubilized in standard SDS Laemmli buffer and maintained at room temperature before loading.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
  • Tissue specificity
    Expressed at variable levels in many human tissues.
  • Involvement in disease
    Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
    Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
  • Sequence similarities
    Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
    • CSE antibody
    • DYT17 antibody
    • DYT18 antibody
    • DYT9 antibody
    • EIG12 antibody
    • erythrocyte/brain antibody
    • Erythrocyte/hepatoma glucose transporter antibody
    • facilitated glucose transporter member 1 antibody
    • Glucose transporter 1 antibody
    • Glucose transporter type 1 antibody
    • Glucose transporter type 1, erythrocyte/brain antibody
    • GLUT antibody
    • GLUT-1 antibody
    • GLUT1 antibody
    • GLUT1DS antibody
    • GLUTB antibody
    • GT1 antibody
    • GTG1 antibody
    • Gtg3 antibody
    • GTR1_HUMAN antibody
    • HepG2 glucose transporter antibody
    • HTLVR antibody
    • Human T cell leukemia virus (I and II) receptor antibody
    • MGC141895 antibody
    • MGC141896 antibody
    • PED antibody
    • RATGTG1 antibody
    • Receptor for HTLV 1 and HTLV 2 antibody
    • SLC2A1 antibody
    • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
    • Solute carrier family 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 1 antibody
    see all

Images

  • ICC/IF image of ab652 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab652, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab652 at 1/250 staining human renal carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed before incubation with the primary antibody for 45 minutes. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • All lanes : Anti-Glucose Transporter GLUT1 antibody (ab652) at 1/1000 dilution

    Lane 1 : 2 week old Mouse brain tissue lysate
    Lane 2 : 2 week old Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat HRP-conjugate anti-rabbit at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab652 staining Glucose Transporter GLUT1 in mouse liver tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and permeabilized with 0.2% Triton X-100 before blocking with 2% BSA was performed for 30 minutes at 200C. The sample was incubated with primary antibody (1/200) for 9 hours at 40C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at dilution at 1/200. DAPI was used to stain the cell nuclei (blue).

    See Abreview

  • ab652 staining Glucose Transporter GLUT1 in rat bone marrow cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed then blocked using 5% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/250 for 9 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/250 dilution. DAPI was used to stain the cell nuclei (blue).

    See Abreview

References

This product has been referenced in:
  • Hu S  et al. Role of angiopoietin-2 in the cardioprotective effect of fibroblast growth factor 21 on ischemia/reperfusion-induced injury in H9c2 cardiomyocytes. Exp Ther Med 14:771-779 (2017). Read more (PubMed: 28672998) »
  • Thackeray JT  et al. Insulin supplementation attenuates cancer-induced cardiomyopathy and slows tumor disease progression. JCI Insight 2:N/A (2017). Read more (PubMed: 28515362) »

See all 89 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse brain, liver, retina, muscle)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
25 µg
Specification
Mouse brain, liver, retina, muscle
Blocking step
Licor Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Jul 20 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (necrotic canine sarcoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization
No
Specification
necrotic canine sarcoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde
Username

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Verified customer

Submitted Dec 18 2015

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (MCF7)
Specification
MCF7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Feb 25 2015

The antibody ab652 binds to a cytoplasmic region of GLUT1.

This antibody has not been purified and therefore its concentration has not been determined. It is a whole antiserum and so we estimate the concentration to be within the range of 1-10 mg/ml.
As for the negative control, I would suggest using an is...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum (goat) 1/50 diluted in PBS BSA5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: buffer citrate
Sample
Human Tissue sections (orbital fat)
Specification
orbital fat
Permeabilization
No
Fixative
Formaldehyde
Username

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Verified customer

Submitted Aug 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Sample
Rat Cell (thyrocytes (FRTL-5))
Specification
thyrocytes (FRTL-5)
Permeabilization
Yes - Saponin 0.1%
Fixative
Paraformaldehyde 4%
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Verified customer

Submitted Aug 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (cells were lysated in Laemmli buffer, gel 10 % acrylamide, nitrocellulose membrane)
Sample
Rat Cell lysate - whole cell (thyrocytes (FRTL-5))
Specification
thyrocytes (FRTL-5)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Aug 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01 M Tris HCL, pH 10
Sample
Mouse Tissue sections (mouse brain tissue)
Specification
mouse brain tissue
Permeabilization
Yes - 0.3% Triton X-100 10 min
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Jun 11 2013

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (2-wk-old mouse brain tissue)
Specification
2-wk-old mouse brain tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Jun 10 2013

1-10 of 58 Abreviews or Q&A

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