Anti-Glucose Transporter GLUT1 antibody, prediluted (ab15310)

Overview

  • Product nameAnti-Glucose Transporter GLUT1 antibody, prediluted
    See all Glucose Transporter GLUT1 primary antibodies
  • Description
    Rabbit polyclonal to Glucose Transporter GLUT1, prediluted
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal).

  • Positive control
    • HepG2 cells, esophagous and breast carcinoma. IF/ICC: HeLa whole cell.

Properties

Applications

Our Abpromise guarantee covers the use of ab15310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IHC-P
  • Application notesIHC-P: Use at an assay dependent dilution. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFacilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
    • Tissue specificityExpressed at variable levels in many human tissues.
    • Involvement in diseaseDefects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
      Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
    • Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
      • CSE antibody
      • DYT17 antibody
      • DYT18 antibody
      • DYT9 antibody
      • EIG12 antibody
      • erythrocyte/brain antibody
      • Erythrocyte/hepatoma glucose transporter antibody
      • facilitated glucose transporter member 1 antibody
      • Glucose transporter 1 antibody
      • Glucose transporter type 1 antibody
      • Glucose transporter type 1, erythrocyte/brain antibody
      • GLUT antibody
      • GLUT-1 antibody
      • GLUT1 antibody
      • GLUT1DS antibody
      • GLUTB antibody
      • GT1 antibody
      • GTG1 antibody
      • Gtg3 antibody
      • GTR1_HUMAN antibody
      • HepG2 glucose transporter antibody
      • HTLVR antibody
      • Human T cell leukemia virus (I and II) receptor antibody
      • MGC141895 antibody
      • MGC141896 antibody
      • PED antibody
      • RATGTG1 antibody
      • Receptor for HTLV 1 and HTLV 2 antibody
      • SLC2A1 antibody
      • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
      • Solute carrier family 2 antibody
      • Solute carrier family 2, facilitated glucose transporter member 1 antibody
      see all

    Anti-Glucose Transporter GLUT1 antibody, prediluted images

    • ICC/IF image of ab15310 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15310, Neat) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • ab15310 staining Glucose Transporter GLUT1 in human esophagous by Immunohistochemistry (FFPE-sections).

    References for Anti-Glucose Transporter GLUT1 antibody, prediluted (ab15310)

    ab15310 has not yet been referenced specifically in any publications.

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