Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)
- Datasheet
- Specific References (57)
- Protocols
Overview
-
Product nameAnti-Glucose Transporter GLUT1 antibody [SPM498]
See all Glucose Transporter GLUT1 primary antibodies -
DescriptionMouse monoclonal [SPM498] to Glucose Transporter GLUT1
-
Host speciesMouse
-
Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-FoFr, WB, IHC-Fr, IHC-Pmore details
-
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic human peptide (the amino acid sequence is considered to be commercially sensitive) (C terminal)
-
Positive control
- HepG2 cells. Esophagous and breast carcinoma.
-
General notesPrevious lots of this antibody gave good results in WB as published in Abreviews. We however are observing multiple bands with recent lots. GLUT1 is a multi-pass membrane protein so we can recommend not boiling the samples in sample buffer. We will also welcome more feedback from successful users.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 2mg/ml BSA, 10mM PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityMonoclonal
-
Clone numberSPM498
-
IsotypeIgG2a
-
Light chain typekappa
-
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Immunohistochemistry kits
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab40084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. Methanol or paraformaldehyde fixed cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF | Use a concentration of 1 - 5 µg/ml. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 21688176 | |
| WB | 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa). Previous lots of this antibody gave good results in WB as published in Abreviews. We however are observing multiple bands with recent lots. GLUT1 is a multi-pass membrane protein so we can recommend heating the samples 60 - 70C for 10 -15 minutes instead of boiling in sample buffer. We will welcome any feedback the successful users have |
|
| IHC-Fr | 1/200. (see Abreview). |
|
| IHC-P | 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
FunctionFacilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
-
Tissue specificityExpressed at variable levels in many human tissues.
-
Involvement in diseaseDefects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia. -
Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
-
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationCell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
-
Database links
- Entrez Gene: 6513 Human
- Entrez Gene: 20525 Mouse
- Entrez Gene: 24778 Rat
- Omim: 138140 Human
- SwissProt: P11166 Human
- SwissProt: P17809 Mouse
- SwissProt: P11167 Rat
- Unigene: 473721 Human
see all -
Alternative names
- Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
- CSE antibody
- DYT17 antibody
see all
Images
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)](http://a.abcam.com/ps/products/40/ab40084/Images/ab40084-254297-ab4008450853.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)Image courtesy of an anonymous AbReview.Immunohistochemical analysis of 10% buffered formalin-fixed paraffin-embedded human dermal carcinoma tissue sections, labelling GLUT1 with ab40084 at a dilution of 1/100 incubated for 12 hours at 4°C. Heat mediated antigen retrival was performed with 10mM sodium citrate buffer at pH 6.0. Blocking was with 5% serum incubated for 1 hour at 21°C. The secondary was a Donkey anti-mouse polyclonal Alexa Fluor® 647 conjugate at 1/200. Counterstaining is DAPI in blue against Nuclear DNA.
-
![Western blot - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)](http://a.abcam.com/ps/products/40/ab40084/Images/Glucose-Transporter-GLUT1-Primary-antibodies-ab40084-4.jpg)
Western blot - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)This image is courtesy of an anonymous AbreviewAnti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084) at 1/500 dilution + Whole cell lysate prepared from human MDA-MB 231 cells at 20 µg
Secondary
HRP conjugated sheep polyclonal to mouse IgG at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 2 minutes
-
![Flow Cytometry - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)](http://a.abcam.com/ps/products/40/ab40084/Images/Glucose-Transporter-GLUT1-Primary-antibodies-ab40084-11.jpg)
Flow Cytometry - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)Overlay histogram showing HeLa cells stained with ab40084 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40084, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)](http://a.abcam.com/ps/products/40/ab40084/Images/Glucose-Transporter-GLUT1-Primary-antibodies-ab40084-5.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)ICC/IF image of ab40084 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40084, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)](http://a.abcam.com/ps/products/40/ab40084/Images/ab40084.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)ab40084 at a 1:200 dilution staining Glucose Transporter GLUT1 in Human esophagous tissue. -
![Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)](http://a.abcam.com/ps/products/40/ab40084/Images/ab40084-219917-ab40084perfusion.jpg)
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)This image is courtesy of an Abreview submitted by Sumit Sarkarab40084 staining Glucose Transporter GLUT1 in mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde and blocked with 20% serum for 20 minutes at room temperature. The sample was incubated with primary antibody (1/200) . A Biotin-conjugated donkey anti-mouse polyclonal (1/200) was used as the secondary antibody.
-
![Western blot - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)](http://a.abcam.com/ps/products/40/ab40084/Images/Glucose-Transporter-GLUT1-Primary-antibodies-ab40084-6.jpg)
Western blot - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)All lanes : Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)
Lane 1 : Cell lysates prepared from NIH3T3 cells
Lane 2 : Cell lysates prepared from MDA MB 231 cells
Lane 3 : Cell lysates prepared from HeLa cells
Predicted band size: 54 kDa4oC (1 freeze/thaw)
-
![Immunohistochemistry (Frozen sections) - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)](http://a.abcam.com/ps/products/40/ab40084/Images/Glucose-Transporter-GLUT1-Primary-antibodies-ab40084-9.jpg)
Immunohistochemistry (Frozen sections) - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)This image is courtesy of an anonymous Abreviewab40084 staining Glucose Transporter GLUT1 in mouse smooth muscle tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 2 hours at 25°C. The sample was incubated with primary antibody (1/200 im PBS-Tween) at 25°C for 1 hour. An Alexa Fluor®594-conjugated Goat polyclonal to mouse IgG (1/250) was used as secondary antibody. -
![Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)](http://a.abcam.com/ps/products/40/ab40084/Images/Glucose-Transporter-GLUT1-Primary-antibodies-ab40084-12.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT1 antibody [SPM498] (ab40084)ICC/IF image of ab40084 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40084, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
This product has been referenced in:
- Kavanagh Williamson M et al. Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1. Viruses 10:N/A (2018). Read more (PubMed: 29518929) »
- Cretella D et al. The anti-tumor efficacy of CDK4/6 inhibition is enhanced by the combination with PI3K/AKT/mTOR inhibitors through impairment of glucose metabolism in TNBC cells. J Exp Clin Cancer Res 37:72 (2018). Read more (PubMed: 29587820) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"