Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715)

Overview

  • Product nameAnti-Glucose Transporter GLUT2 antibody [5D1]
    See all Glucose Transporter GLUT2 primary antibodies
  • Description
    Mouse monoclonal [5D1] to Glucose Transporter GLUT2
  • Specificityab85715 recognizes Glucose Transporter GLUT2 expression on hGlut2-transfected NS0 cells but not on control transfectants. Detects Glut2 on the surface of the Human intestinal cell line Caco2 in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with hGlut1 or hGlut3.
  • Tested applicationsSuitable for: IHC-P, ICC, Flow Cyt, IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human Glucose Transporter GLUT2 transfected NS0 cells.

  • Positive control
    • Liver tissue.

Applications

Our Abpromise guarantee covers the use of ab85715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
ICC Use a concentration of 10 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21857924
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

Target

  • FunctionFacilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.
  • Tissue specificityLiver, insulin-producing beta cell, small intestine and kidney.
  • Involvement in diseaseFanconi-Bickel syndrome
  • Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational
    modifications
    N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • liver antibody
    • Glucose Transporter 2 antibody
    • Glucose Transporter GLUT2 antibody
    • Glucose transporter type 2 antibody
    • Glucose transporter type 2 liver antibody
    • Glucose transporter, liver/islet antibody
    • GLUT-2 antibody
    • GLUT2 antibody
    • GTR2_HUMAN antibody
    • GTT2 antibody
    • SLC2A2 antibody
    • Solute carrier family 2 (facilitated glucose transporter) member 2 antibody
    • Solute carrier family 2 facilitated glucose transporter member 2 antibody
    • Solute carrier family 2, facilitated glucose transporter member 2 antibody
    see all

Anti-Glucose Transporter GLUT2 antibody [5D1] images

  • ab85715, at 10 µg/ml, staining Glucose Transporter GLUT2 in formalin-fixed, paraffin-embedded human Liver tissue by Immunohistochemistry.
  • Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 57 kDa
    Observed band size : 57 kDa


    Exposure time : 8 minutes
  • Overlay histogram showing HepG2 cells stained with ab85715 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85715, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemical analysis of frozen pancreatic tissue in control (left) or TAT-MafA mutant (right) neonate mice.

    Glucose Transporter GLUT2 was stained using ab85715 (green), at 10 mg/ml. An AlexaFluor®647-conjugated donkey anti-mouse IgG was used as the secondary antibody.

References for Anti-Glucose Transporter GLUT2 antibody [5D1] (ab85715)

This product has been referenced in:
  • Mano Y  et al. Correlation between biological marker expression and fluorine-18 fluorodeoxyglucose uptake in hepatocellular carcinoma. Am J Clin Pathol 142:391-7 (2014). Human . Read more (PubMed: 25125631) »
  • Kern M  et al. Liver-restricted Repin1 deficiency improves whole-body insulin sensitivity, alters lipid metabolism, and causes secondary changes in adipose tissue in mice. Diabetes 63:3295-309 (2014). Read more (PubMed: 24760138) »

See all 3 Publications for this product

Product Wall

Thank you for your reply and also for checking the lysate. This is really helpful.

I am sorry to hear that the problem has not been resolved.
Since you see no protein bands before transfer, it does not surprise me that you see none after...

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Thank you for contacting us.
I am sorry to hear about the problem the pancreas lysate is causing you.

As we discussed over the phone, it would be worthwhile trying the following in order to determine the cause of the problem:

...

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Vielen Dank für Ihre Anfrage.

Leider haben wir keinen Antikörper, der allen genannten Anforderungen entspricht.

Wir führen einen Maus-monoklonalen GLUT2-Antikörper, der bislang nur für humane Proben getestet wurde (ab85715). Das I...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Pancreas)
Specification Pancreas
Fixative Paraformaldehyde
Permeabilization Yes - PBST (0.02% Tween)
Blocking step (agent) for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Mr. Tirosh Shapira

Verified customer

Submitted Mar 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"