The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.
Liver, insulin-producing beta cell, small intestine and kidney.
Involvement in disease
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.
ab111117, at 2.5µg/ml, staining Glucose Transporter GLUT2 in formalin-fixed, paraffin-embedded Human Liver tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-Glucose Transporter GLUT2 antibody (ab111117)Image courtesy of Prof Ahter Dilsad Sanlioglu by Abreview.
ab111117 staining Glucose Transporter GLUT2 in human MCF7 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, blocked with 1% BSA for 30 minutes at 22°C and then incubated with ab111117 at a 1/200 dilution. The secondary used was ab7123, Donkey polyclonal Secondary Antibody to Goat IgG - H&L (TR), pre-adsorbed, used at a 1/200 dilution.
Blanco AM et al. Ghrelin Facilitates GLUT2-, SGLT1- and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus). Sci Rep7:45024 (2017).
Read more (PubMed: 28338019) »
Kahraman S et al. Diabetes-resistant NOR mice are more severely affected by streptozotocin compared to the diabetes-prone NOD mice: correlations with liver and kidney GLUT2 expressions. J Diabetes Res2015:450128 (2015).
Read more (PubMed: 25699277) »