Anti-Glucose Transporter GLUT4 antibody [1F8] (ab35826)
Key features and details
- Mouse monoclonal [1F8] to Glucose Transporter GLUT4
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-Glucose Transporter GLUT4 antibody [1F8]
See all Glucose Transporter GLUT4 primary antibodies -
Description
Mouse monoclonal [1F8] to Glucose Transporter GLUT4 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Rat Glucose Transporter GLUT4. Partially purified vesicles containing insulin-responsive glucose transporter 4.
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Epitope
This antibody has been shown to recognise an epitope in the cytoplasmic portion of the molecule. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
1F8 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab35826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
5 µg/ml of antibody will detect Glucose Transporter protein contained in 50-200 µg of solubilised membrane. |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa. 5 µg/ml of antibody will detect Glucose Transporter protein contained in 50-200 µg of solubilised membrane. |
Target
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Function
Insulin-regulated facilitative glucose transporter. -
Tissue specificity
Skeletal and cardiac muscles; brown and white fat. -
Involvement in disease
Diabetes mellitus, non-insulin-dependent -
Sequence similarities
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
Post-translational
modificationsSumoylated. -
Cellular localization
Cell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration. - Information by UniProt
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Database links
- Entrez Gene: 20528 Mouse
- SwissProt: P14142 Mouse
- Unigene: 10661 Mouse
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Alternative names
- insulin-responsive antibody
- Glucose transporter GLUT 4 antibody
- Glucose transporter type 4 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (12)
ab35826 has been referenced in 12 publications.
- Rahimi R et al. TMF1 is upregulated by insulin and is required for a sustained glucose homeostasis. FASEB J 35:e21295 (2021). PubMed: 33475194
- Li H et al. Mixed conjugated linoleic acid sex-dependently reverses high-fat diet-induced insulin resistance via the gut-adipose axis. FASEB J 35:e21466 (2021). PubMed: 33734496
- Lee K et al. Effect of Dietary Silk Peptide on Obesity, Hyperglycemia, and Skeletal Muscle Regeneration in High-Fat Diet-Fed Mice. Cells 9:N/A (2020). PubMed: 32041272
- Al Koborssy D et al. Modulation of olfactory-driven behavior by metabolic signals: role of the piriform cortex. Brain Struct Funct 224:315-336 (2019). PubMed: 30317390
- Sociali G et al. Pharmacological Sirt6 inhibition improves glucose tolerance in a type 2 diabetes mouse model. FASEB J 31:3138-3149 (2017). PubMed: 28386046