Anti-Glucose Transporter GLUT4 antibody (ab654)


  • Product nameAnti-Glucose Transporter GLUT4 antibody
    See all Glucose Transporter GLUT4 primary antibodies
  • Description
    Rabbit polyclonal to Glucose Transporter GLUT4
  • SpecificityGLUT4.
  • Tested applicationsSuitable for: ICC/IF, WB, IP, Immunomicroscopy, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig, Armenian hamster
  • Immunogen

    Synthetic peptide corresponding to Rat Glucose Transporter GLUT4 aa 1-15 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab115831)

  • Positive control
    • IHC-P: FFPE human heart tissue sections.



Our Abpromise guarantee covers the use of ab654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB 1/2000 - 1/2500. Detects a band of approximately 45 kDa (predicted molecular weight: 54.8 kDa).Can be blocked with Rat Glucose Transporter GLUT4 peptide (ab115831).

The samples MUST NOT be boiled before running the gel as highly hydrophobic membrane proteins, like glucose transporters, aggregate severely at high temperatures due to the hydrophobic effect, which increases as a direct function of temperature. If the samples are boiled, much of the transporter will aggregate so severely it doesn't enter the running gel and higher order oligomers will be observed.

It is recomended to use 3% milk block.

IP Use at an assay dependent concentration. PubMed: 18234908
Immunomicroscopy Use at an assay dependent concentration. Can also can be used for Immunogold EM (purified IgG). See references for specific protocols.
IHC-Fr 1/500.
IHC-P Use at an assay dependent concentration.


  • FunctionInsulin-regulated facilitative glucose transporter.
  • Tissue specificitySkeletal and cardiac muscles; brown and white fat.
  • Involvement in diseaseDiabetes mellitus, non-insulin-dependent
  • Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
  • Post-translational
  • Cellular localizationCell membrane. Endomembrane system. Cytoplasm, perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
  • Information by UniProt
  • Database links
  • Alternative names
    • insulin-responsive antibody
    • Glucose transporter GLUT 4 antibody
    • Glucose transporter type 4 antibody
    • Glucose transporter type 4 insulin responsive antibody
    • GLUT 4 antibody
    • GLUT-4 antibody
    • GLUT4 antibody
    • GTR4_HUMAN antibody
    • Insulin responsive glucose transporter type 4 antibody
    • kug antibody
    • SLC 2A4 antibody
    • SLC2A4 antibody
    • solute carrier family 2 (facilitated glucose transporter) member 4 antibody
    • Solute carrier family 2 member 4 antibody
    • Solute carrier family 2, facilitated glucose transporter member 4 antibody
    see all

Anti-Glucose Transporter GLUT4 antibody images

  • Anti-Glucose Transporter GLUT4 antibody (ab654) at 1/1000 dilution (Diluted with TBST and Incubated for 16 hours at 4°C) + Mouse Heart at 30 µg with Milk, 1 hour at 25°C at 3 %

    Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) HRP at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 54.8 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 seconds

    Image courtesy of an anonymous Abreview

    See Abreview

  • IHC image of Glucose Transporter GLUT4 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab654, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ab654 staining GLUT4 in human mesenchymal stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/100 in 10% Horse serum) for 1 hour at 22°C. A FITC-conjugated rabbit polyclonal was used as the secondary antibody (1/100).

    See Abreview

  • ab654 at a 1/300 dilution, staining human GLUT4 in fetal heart tissue by Immunohistochemistry, frozen tissue.

    See Abreview

  • ICC/IF image of ab654 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab654, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab654 staining GLUT4 in human endometriosis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% BSA for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in 3% BSA in TBS) for 16 hours at 4°C. An undiluted Biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab654 at 1/500 dilution staining GLUT4 in mouse muscle by immunohistochemistry (frozen sections). Sections were methanol fixed prior to blocking in 5% goat serum for 1 hour at 25°C and then incubated with ab654 for 1 hour at 25°C. A goat polyclonal to rabbit IgG, diluted 1/500, was used as the secondary antibody.

    See Abreview

References for Anti-Glucose Transporter GLUT4 antibody (ab654)

This product has been referenced in:
  • Lu H  et al. Transient hypoxia reprograms differentiating adipocytes for enhanced insulin sensitivity and triglyceride accumulation. Int J Obes (Lond) 40:121-8 (2016). Mouse . Read more (PubMed: 26219415) »
  • Zhou Y  et al. MicroRNA-29a induces insulin resistance by targeting PPARd in skeletal muscle cells. Int J Mol Med 37:931-8 (2016). WB ; Rat . Read more (PubMed: 26936652) »

See all 68 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse brain, heart, liver, muscle, and retina)
Gel Running Conditions Reduced Denaturing (12% gel)
Loading amount 30 µg
Specification Mouse brain, heart, liver, muscle, and retina
Blocking step Licor Odyssey Blocking BUffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 23 2016

Application Western blot
Sample Mouse Tissue lysate - whole (heart)
Gel Running Conditions Reduced Denaturing (gel 4-15% SDS)
Loading amount 30 µg
Specification heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 04 2015

I would recommend looking at the human protein atlas which shows in which tissues the protein is expressed:

From this, it would seem that the following tissues could be good negative ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample Human Tissue sections (Endometriosis)
Specification Endometriosis
Permeabilization No
Fixative Paraformaldehyde

Dr. Brett Mckinnon

Verified customer

Submitted Jun 10 2013

Hello and thank you for sending your image. We do highly recommend boiling your samples with this antibody, and this may reduce background. However, this target is phosphorylated and glycosylated which is another reason you may see two strong bands. If...

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Thank you for your new contact. My colleague is out of office today and she has asked me to look after her customers.

Currently, we do not have a different Western blot image available.The predicted molecular weight of GLUT4 is54.8 kDa, howev...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Mesenchymal stem cells)
Specification Mesenchymal stem cells
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C

Mr. Steven Johnstone

Verified customer

Submitted Sep 11 2012

Thank you for contacting us.

All the images from WB assay in the datasheet belong to Abreviews from other users. I am afraid we do not have access to the complete images.

As new images or references are brought to our attention, the...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for your enquiry.

I can confirm as discussed on the telephone that ab654 Glucose Transporter GLUT4 antibody is sold as whole antiserum. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture superna...

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