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Glucose Uptake Assay Kit (Colorimetric) (ab136955) is highly sensitive and easy to use non-radioactive kit which can detect glucose uptake as low as 10 pmol/well in a variety of cell types. Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells. In this assay, the 2-DG6P is oxidized to generate NADPH, which can be determined by an enzymatic recycling amplification reaction.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
|2-Deoxyglucose||Purple||1 x 1ml|
|2-DG6P Standard (Lyophilized)||Yellow||1 vial|
|Assay Buffer||WM||1 x 25ml|
|Enzyme Mix (Lyophilized)||Orange||1 vial|
|Extraction Buffer||NM||1 x 17ml|
|Glutathione Reductase (Lyophilized)||Green||2 vials|
|Neutralizing Buffer||Clear||1 x 2.5ml|
|Recycling Mix(Lyophilized)||Blue||1 vial|
Our Abpromise guarantee covers the use of ab136955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"