Anti-Glucosidase 2 subunit beta antibody (ab96062)

Overview

  • Product nameAnti-Glucosidase 2 subunit beta antibody
    See all Glucosidase 2 subunit beta primary antibodies
  • Description
    Rabbit polyclonal to Glucosidase 2 subunit beta
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment within Human Glucosidase 2 subunit beta aa 1-253. The exact sequence is proprietary.

  • Positive control
    • A431 whole cell lysate, A549 cells This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7.

Properties

Applications

Our Abpromise guarantee covers the use of ab96062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionRegulatory subunit of glucosidase II.
  • PathwayGlycan metabolism; N-glycan metabolism.
  • Involvement in diseaseDefects in PRKCSH are a cause of polycystic liver disease (PCLD) [MIM:174050]. PCLD is an autosomal dominant disorder and is characterized by the presence of multiple liver cysts of biliary epithelial origin. PCLD is a distinct clinical and genetic entity that can occur independently from autosomal dominant polycystic kidney disease (ADPKD) [MIM:173900], which in a considerable but uncertain proportion of cases is associated with hepatic cysts.
  • Sequence similaritiesContains 2 EF-hand domains.
    Contains 1 PRKCSH domain.
  • Cellular localizationEndoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • 80K-H protein antibody
    • AGE-binding receptor 2 antibody
    • AGE-R2 antibody
    • G19P1 antibody
    • GLU2B_HUMAN antibody
    • Glucosidase 2 subunit beta antibody
    • Glucosidase II beta subunit antibody
    • Glucosidase II subunit beta antibody
    • Hepatocystin antibody
    • PCLD antibody
    • PKCSH antibody
    • PLD1 antibody
    • PRKCSH antibody
    • Protein kinase C substrate 60.1 kDa protein heavy chain antibody
    • Protein kinase C substrate 80 Kda protein antibody
    • Protein kinase C substrate 80K-H antibody
    • Protein kinase C substrate, 80 Kda protein antibody
    see all

Anti-Glucosidase 2 subunit beta antibody images

  • ICC/IF image of ab96062 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96062 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Glucosidase 2 subunit beta antibody (ab96062) at 1/10000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 59 kDa
    7.5% SDS PAGE
  • ab96062 at 1/200 dilution staining Glucosidase 2 subunit beta in paraformaldehyde-fixed A549 cells by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). In the lower image, the cells were costained with Hoechst 33342.

References for Anti-Glucosidase 2 subunit beta antibody (ab96062)

ab96062 has not yet been referenced specifically in any publications.

Product Wall

DISCOUNT CODE: ****** Expiration date: ******I am very pleased to hear you would like to accept our offer and test ab96062 in [UNTESTED SPECIES/APP]. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, plea...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"