The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
RelevanceGlucuronic Acid Epimerase (GLCE) is responsible for epimerization of D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) of Heparan sulfate (HS), which endows the nascent polysaccharide chain with the ability to bind growth factors and cytokines (Ghiselli and Agrawal, 2005 [PubMed 15853773]).
Cellular localizationGolgi apparatus membrane; Single-pass type II membrane protein