The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/6000 - 1/24000.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.
FunctionMay be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
Involvement in diseaseDefects in GLUD1 are the cause of familial hyperinsulinemic hypoglycemia type 6 (HHF6) [MIM:606762]; also known as hyperinsulinism-hyperammonemia syndrome (HHS). Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. In HHF6 elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.
Sequence similaritiesBelongs to the Glu/Leu/Phe/Val dehydrogenases family.
Human Pancreas (formalin fixed, paraffin embedded tissue) stained with ab116714 at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-GLUD1 antibody (ab116714)
has not yet been referenced specifically in any publications.
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