Overview

  • Product name
  • Description
    Rabbit polyclonal to GLUD1
  • Specificity
    Due to high sequence homology with GLUD2 there is a potential for cross-reactivity.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human GLUD1 aa 64-366.

  • Positive control
    • A549, HepG2 and HCT116 cell lysates; HeLa cells; Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
  • Involvement in disease
    Defects in GLUD1 are the cause of familial hyperinsulinemic hypoglycemia type 6 (HHF6) [MIM:606762]; also known as hyperinsulinism-hyperammonemia syndrome (HHS). Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. In HHF6 elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.
  • Sequence similarities
    Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI118167 antibody
    • DHE3_HUMAN antibody
    • EC 1.4.1.3 antibody
    • GDH 1 antibody
    • GDH antibody
    • Gdh-X antibody
    • GDH1 antibody
    • GLUD antibody
    • Glud1 antibody
    • Glud1a antibody
    • Glud1b antibody
    • Gludl antibody
    • Glutamate dehydrogenase (NAD(P)+) antibody
    • Glutamate dehydrogenase 1 antibody
    • Glutamate dehydrogenase 1 mitochondrial antibody
    • Glutamate dehydrogenase 1a antibody
    • Glutamate dehydrogenase 1b antibody
    • Memory-related gene 2 protein antibody
    • MGC127177 antibody
    • MGC132003 antibody
    • MGC80801 antibody
    • MGC93608 antibody
    • mitochondrial antibody
    • MRG-2 antibody
    see all

Images

  • Anti-GLUD1 antibody (ab153973) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 61 kDa
    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling GLUD1 with ab153973 at 1/100 dilution. Lower panel co-stained with Hoechst 33342.

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling GLUD1 with ab153973 at 1/250 dilution.

References

This product has been referenced in:
  • Kim S  et al. Expression of glutamine metabolism-related proteins according to molecular subtype of breast cancer. Endocr Relat Cancer 20:339-48 (2013). Read more (PubMed: 23507704) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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