Recombinant
RabMAb

Anti-GLUD1 antibody [EPR11370] (ab168352)

Overview

  • Product name
    Anti-GLUD1 antibody [EPR11370]
    See all GLUD1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11370] to GLUD1
  • Specificity
    Ab168352 immunogen shows 93% identity to human GLUD2, therefore there exists a potential for cross-reactivity.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GLUD1 aa 500 to the C-terminus. The exact sequence is proprietary.
    Database link: P00367

  • Positive control
    • WB: Human fetal liver tissue lysate and HepG2 cell lysate. IHC-P: Human heart and liver tissues. ICC/IF: HepG2 cells. Flow Cyt: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab168352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/2000.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.
    • Involvement in disease
      Defects in GLUD1 are the cause of familial hyperinsulinemic hypoglycemia type 6 (HHF6) [MIM:606762]; also known as hyperinsulinism-hyperammonemia syndrome (HHS). Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. In HHF6 elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.
    • Sequence similarities
      Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI118167 antibody
      • DHE3_HUMAN antibody
      • EC 1.4.1.3 antibody
      • GDH 1 antibody
      • GDH antibody
      • Gdh-X antibody
      • GDH1 antibody
      • GLUD antibody
      • Glud1 antibody
      • Glud1a antibody
      • Glud1b antibody
      • Gludl antibody
      • Glutamate dehydrogenase (NAD(P)+) antibody
      • Glutamate dehydrogenase 1 antibody
      • Glutamate dehydrogenase 1 mitochondrial antibody
      • Glutamate dehydrogenase 1a antibody
      • Glutamate dehydrogenase 1b antibody
      • Memory-related gene 2 protein antibody
      • MGC127177 antibody
      • MGC132003 antibody
      • MGC80801 antibody
      • MGC93608 antibody
      • mitochondrial antibody
      • MRG-2 antibody
      see all

    Images

    • ab168352 showing +ve staining in Human normal pancreas.
    • Immunofluorescent analysis of HepG2 cells labeling Glutamate Dehydrogenase with ab168352 at 1/50 dilution.
    • All lanes : Anti-GLUD1 antibody [EPR11370] (ab168352) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 61 kDa
    • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GLUD1 (red) with ab168352 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    • ab168352 showing +ve staining in Human normal uterus.
    • ab168352 showing +ve staining in Human normal kidney.
    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling Glutamate Dehydrogenase with ab168352 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Glutamate Dehydrogenase with ab168352 at 1/100 dilution.

    References

    ab168352 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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