The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa.Can be blocked with GLUT9 peptide (ab105952). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionTransport urate and fructose. May have a role in the urate reabsorption by proximal tubules. Also transports glucose at low rate.
Tissue specificityMost strongly expressed in basolateral membranes of proximal renal tubular cells, liver and placenta. Also detected in lung, blood leukocytes, heart skeletal muscle and chondrocytes from articular cartilage. Isoform 2 is only detected in the apical membranes of polarized renal tubular cells and placenta. Isoform 1 and isoform 2 are detected in kidney membrane (at protein level).
Involvement in diseaseHypouricemia renal 2
Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
Solute carrier family 2 (facilitated glucose transporter) member 9 antibody
Solute carrier family 2 facilitated glucose transporter member 9 antibody
Solute carrier family 2 member 9 antibody
Solute carrier family 2 member 9 protein antibody
Solute carrier family 2, facilitated glucose transporter member 9 antibody
Urate voltage driven efflux transporter 1 antibody
Anti-GLUT9 antibody images
Western blot - GLUT9 antibody (ab82910)
Anti-GLUT9 antibody (ab82910) at 1 µg/ml (5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 59 kDa Observed band size : 59 kDa
References for Anti-GLUT9 antibody (ab82910)
This product has been referenced in:
Karim S et al. Dysregulated hepatic expression of glucose transporters in chronic disease: contribution of semicarbazide-sensitive amine oxidase to hepatic glucose uptake. Am J Physiol Gastrointest Liver Physiol307:G1180-90 (2014).
Read more (PubMed: 25342050) »
Gaccioli F et al. Maternal overweight induced by a diet with high content of saturated fat activates placental mTOR and eIF2 alpha signaling and increases fetal growth in rats. Biol Reprod89:96 (2013).
Read more (PubMed: 24006279) »