Overview

  • Product nameGlutamate Assay Kit
    See all Glutamate kits
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Cell Lysate
  • Assay typeQuantitative
  • Assay time
    0h 40m
  • Product overview

    Glutamate Assay Kit (ab83389) provides a sensitive detection method of the glutamate in a variety of samples. This kit will only measure free glutamate levels but not glutamic acid found in the backbone of peptides or proteins. The glutamate Enzyme Mix recognizes glutamate as a specific substrate leading to proportional color development.The amount of glutamate can therefore be easily quantified by colorimetric spectrophotometry at OD = 450 nm.

     

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Glutamate is the most abundant swift excitatory neurotransmitter in the mammalian nervous system. It is believed to be involved in learning and memory and has appeared to be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, some forms of mental retardation and Alzheimer's disease. Glutamic acid is also present in a wide variety of foods, and has been used as a flavor enhancer in food industry.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • RelevanceGlutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Glutamate is the most abundant swift excitatory neurotransmitter in the mammalian nervous system. It is believed to be involved in learning and memory and has appeared to be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, some forms of mental retardation and Alzheimer's disease. Glutamic acid is also present in a wide variety of foods, and has been used as a flavor enhancer in food industry.

Applications

Our Abpromise guarantee covers the use of ab83389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Glutamate Assay Kit images

  • Example of Glutamate standard curve obtained using ab83389

Protocols

References for Glutamate Assay Kit (ab83389)

This product has been referenced in:
  • Xue M  et al. Supramolecular Probes for Assessing Glutamine Uptake Enable Semi-Quantitative Metabolic Models in Single Cells. J Am Chem Soc 138:3085-93 (2016). Read more (PubMed: 26916347) »
  • Evonuk KS  et al. Inhibition of System Xc(-) Transporter Attenuates Autoimmune Inflammatory Demyelination. J Immunol 195:450-63 (2015). Read more (PubMed: 26071560) »

See all 5 Publications for this product

Product Wall

Glutamate levels in PMN supernatants

Good Excellent 5/5 (Ease of Use)
Abreviews
I wanted to analyze free glutamate in the supernatant of short-term stimulated human blood granulocytes of healthy donors and two groups of clinic patients. The cells were freshly isolated from human whole blood and stimulated for 60-70 minutes at 37 °C in HBSS supplemented with 1.6 mM calcium chloride. Cell-free supernatants were snap-frozen as indicated in the assay protocol and used undiluted in the assay including a HBSS background control. Unfortunately, measured glutamate levels were very low.
The assay protocol is good, the assay is easy and quick to perform.
Username

Abcam user community

Verified customer

Submitted Apr 25 2017

Abreviews
I used lysates of mouse hippocampus. Since the "enzyme" is present in fresh lysates, deproteinization is required
http://www.abcam.com/protocols/deproteinization-protocol
Don't forget to leave aside sample for Bradford normalization before deproteinization
Username

Dr. Tamar Licht

Verified customer

Submitted Mar 08 2016



I have contacted the lab and they have confirmed that we can add the Glutamate assay buffer to the catalogue to purchase separately to dilute your samples.

However, the lab have also said that you can simply dilute yoursamples in dei...

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Préparation de plasma:

Prélever le sang dans un tube de « sodium heparin » (par exemple BD Vacutainer, Cat No:. 8001302 ou 16852)
Centrifuger pendant 10 minutes à 3000 rpm
Faire des aliquots e...

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Unfortunately neither of these kits will be able to detect glutamic acid in peptides/proteins. ab83389 only measures free glutamate levels not glutamic acid and ab138883 is based on enzymatic reactions so it is most likely that it will not react with t...

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Non normalement les ions NH3 ne devraient pas interférer le dosage avec ce kit.



The Glutamate Assay Kit (ab83389) can be used to asses the level of glutamate in a range of different biological samples. Unfortunately, the dilution range required will need to be assessed for the different samples you are hoping to use the ...

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I've checked with the laboratory, and this kit is very specific for glutamate only. Glutamine is not going to cross react and produce false signals. So, no optimization is required and the glutamine media will be fine.

J’ai eu une réponse du laboratoire concernant la préparation de vos échantillons. Les composants de votre tampon ne devraient pas causer d’interférence avec ce kit. Cependant, le laboratoire recommande de diluer ...

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Thank you for contacting us.

After doing some research on this subject it seems that astrocytes release glutamate in a Ca2+ dependent manner. It is a very well-known function/property of astrocytes involving transmitter uptake and release. Read More

1-10 of 37 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"