Glutamate Dehydrogenase Activity Assay Kit (ab102527)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.01 mU/well
Overview
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Product name
Glutamate Dehydrogenase Activity Assay Kit
See all Glutamate Dehydrogenase kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Enzyme activity -
Sensitivity
> 0.01 mU/well -
Product overview
Glutamate Dehydrogenase Activity Assay Kit (ab102527) provides a convenient tool for sensitive detection of GDH in a variety of samples. GDH in sample will consume glutamate as a specific substrate and generate NADH stoichiometrically, resulting in a proportional color development. The GDH activity is easily quantified colorimetrically (λ = 450 nm). This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.
Visit our FAQs page for tips and troubleshooting. -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K729 Glutamate Dehydrogenase Activity Colorimetric Assay Kit. K729-100 is the same size as the 100 test size of ab102527.
Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to α-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Developer Solution III 1 vial GDH Assay Buffer 1 x 25ml GDH Positive Control 1 vial Glutamate Solution 1 x 1ml NADH Standard I 1 vial -
Research areas
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Relevance
Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to alpha-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes. -
Alternative names
- GDH
Images
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab102527 has been referenced in 22 publications.
- John A et al. Alterations in Energy Metabolism, Mitochondrial Function and Redox Homeostasis in GK Diabetic Rat Tissues Treated with Aspirin. Life (Basel) 12:N/A (2022). PubMed: 35054496
- Zhou W et al. SENP1-Sirt3 signaling promotes a-ketoglutarate production during M2 macrophage polarization. Cell Rep 39:110660 (2022). PubMed: 35417703
- Lee SB & Yang HO Sinapic Acid Ameliorates REV-ERB a Modulated Mitochondrial Fission against MPTP-Induced Parkinson's Disease Model. Biomol Ther (Seoul) 30:409-417 (2022). PubMed: 35611585
- Weightman Potter PG et al. Glutamate Prevents Altered Mitochondrial Function Following Recurrent Low Glucose in Hypothalamic but Not Cortical Primary Rat Astrocytes. Cells 11:N/A (2022). PubMed: 36359822
- Meszaros AT et al. Mitochondrial respiration during normothermic liver machine perfusion predicts clinical outcome. EBioMedicine 85:104311 (2022). PubMed: 36374770