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Glutamate Dehydrogenase Activity Assay Kit (ab102527) provides a convenient tool for sensitive detection of GDH in a variety of samples. GDH in sample will consume glutamate as a specific substrate and generate NADH stoichiometrically, resulting in a proportional color development. The GDH activity is easily quantified colorimetrically (λ = 450 nm). This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.
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Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to α-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes.
|GDH Assay Buffer||WM||1 x 25ml|
|GDH Developer||Red||1 vial|
|GDH Positive Control||Green||1 vial|
|Glutamate||Blue||1 x 1ml|
Our Abpromise guarantee covers the use of ab102527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|