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Glutamate Dehydrogenase Detection Kit (ab102527) provides a convenient tool for sensitive detection of GDH in a variety of samples. GDH in sample will consume glutamate as a specific substrate and generate NADH stoichiometrically, resulting in a proportional color development. The GDH activity is easily quantified colorimetrically (? = 450 nm). This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.
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Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to a-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes.
|GDH Assay Buffer||WM||1 x 25ml|
|GDH Developer||Red||1 vial|
|GDH Positive Control||Green||1 vial|
|Glutamate||Blue||1 x 1ml|
Our Abpromise guarantee covers the use of ab102527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
GDH measured showing activity (mU) per mL of tested sample
GDH measured in cell lysates showing activity (mU) per 106 cells tested
GDH measured in various samples showing O.D.'s in time course
ab102527 has not yet been referenced specifically in any publications.