• Product nameGlutamate Dehydrogenase Detection Kit
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Sensitivity
    > 0.01 mU/well
  • Product overview

    Glutamate Dehydrogenase Detection Kit (ab102527) provides a convenient tool for sensitive detection of GDH in a variety of samples. GDH in sample will consume glutamate as a specific substrate and generate NADH stoichiometrically, resulting in a proportional color development. The GDH activity is easily quantified colorimetrically (? = 450 nm). This assay detects GDH activity as low as 0.01mU in serum or tissue and cell extracts.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Glutamate dehydrogenase (GDH) is an enzyme that converts glutamate to a-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes.

  • Tested applicationsSuitable for: Functional Studiesmore details


  • RelevanceGlutamate dehydrogenase (GDH) is an enzyme that converts glutamate to alpha-Ketoglutarate, and vice versa. It represents a key link between catabolic and metabolic pathways, and is therefore ubiquitous in eukaryotes.
  • Alternative names
    • GDH


Our Abpromise guarantee covers the use of ab102527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Glutamate Dehydrogenase Detection Kit images

  • GDH measured showing activity (mU) per mL of tested sample

  • GDH measured in cell lysates showing activity (mU) per 106 cells tested

  • GDH measured in various samples showing O.D.'s in time course

  • NADH Standard Curve


References for Glutamate Dehydrogenase Detection Kit (ab102527)

This product has been referenced in:
  • Leite NC  et al. Protein malnutrition potentiates the amplifying pathway of insulin secretion in adult obese mice. Sci Rep 6:33464 (2016). Functional Studies . Read more (PubMed: 27633083) »

See 1 Publication for this product

Product Wall

- No special preparation is required for cell culture supernatant. Simply collect the media and use in the assay. It is worth testing different dilutions with GDH assay buffer. I would suggest using 5-50 ul as with serum samples, bringing the final vol...

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Thank you for contacting Abcam regarding ab102527.

We have not tested this kit with lactobacilli, but theoretically it should work. Further protocol optimization may be necessary for best results.

Unfortunately, we do no...

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Thank you for your patience.

Unfortunately, the species protein is propriety and I cannot give you this information. However, the lab just confirmed that this kitab102527 Glutamate Dehydrogenase DetectionKitis suitable for sheep serum.

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Thank you for contacting us.

Unfortunately, we just have the aforementioned kit you mentioned butnothing regarding the other enzymes.

As the species is not given, I assumed that the test was established using the human protein. I c...

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