Anti-Glutamate Receptor 1 (AMPA subtype) antibody (ab31232)

Overview

  • Product name
    Anti-Glutamate Receptor 1 (AMPA subtype) antibody
    See all Glutamate Receptor 1 (AMPA subtype) primary antibodies
  • Description
    Rabbit polyclonal to Glutamate Receptor 1 (AMPA subtype)
  • Specificity
    Based on sequence analysis, ab31232 shares 100% homology with Human, Mouse and Rat Glutamate Receptor 1 (AMPA subtype), however we have been unable to demonstrate reactivity with human tissues in experiments. Abcam welcomes customer feedback and would appreciate any comments regarding this product.
  • Tested applications
    Suitable for: WB, IHC-FoFr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Common marmoset
    Predicted to work with: Human, Monkey
  • Immunogen

    Synthetic peptide derived from within residues 850 to the C-terminus of Human Glutamate Receptor 1 (AMPA subtype).

    (Peptide available as ab28424.)

  • Positive control
    • Mouse and Rat Brain Tissue Lysates;

Properties

Applications

Our Abpromise guarantee covers the use of ab31232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa.Can be blocked with Human Glutamate Receptor 1 (AMPA subtype) peptide (ab28424).
IHC-FoFr Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
  • Tissue specificity
    Widely expressed in brain.
  • Sequence similarities
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
  • Post-translational
    modifications
    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • Cellular localization
    Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
  • Information by UniProt
  • Database links
  • Alternative names
    • GLUR 1 antibody
    • GLUR A antibody
    • AMPA 1 antibody
    • AMPA selective glutamate receptor 1 antibody
    • AMPA-selective glutamate receptor 1 antibody
    • GluA1 antibody
    • GLUH1 antibody
    • GluR K1 antibody
    • GluR-1 antibody
    • GluR-A antibody
    • GluR-K1 antibody
    • GLUR1 antibody
    • GLURA antibody
    • GluRK1 antibody
    • Glutamate receptor 1 antibody
    • Glutamate receptor ionotropic AMPA 1 antibody
    • Glutamate receptor ionotropic antibody
    • Glutamate receptor, ionotropic, AMPA 1 antibody
    • Gria1 antibody
    • GRIA1_HUMAN antibody
    • HBGR1 antibody
    • MGC133252 antibody
    • OTTHUMP00000160643 antibody
    • OTTHUMP00000165781 antibody
    • OTTHUMP00000224241 antibody
    • OTTHUMP00000224242 antibody
    • OTTHUMP00000224243 antibody
    see all

Images

  • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) antibody (ab31232) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : 102 kDa
    Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab31232 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Immunohistochemistical detection of Glutamate Receptor 1 (AMPA subtype)  with ab31232 on formaldehyde-fixed paraffin-embedded rat cerebellum sections. Antigen retrieval step: heat mediated  in citric acid pH6. Blocking step: 1% BSA  for 10 mins @ rt°C.  Primary antibody was incubated at 1/200 for 2 hours @ room temp. Secondary antibody: anti-rabbit IgGs conjugated to biotin (1/200). The composite image shows localisation of this protein apparently exclusive to the molecular layer of the cerebellum upper image (low power). In the lower image (higher power), the molecular layer positivity appears to be compartmentalised/restricted.

    See Abreview

  • ab31232 staining Glutamate Receptor 1 in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/3000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary. 

    See Abreview

  • Immunohistochemistical detection of Glutamate Receptor 1 (AMPA subtype) with ab31232 on  formaldehyde-fixed paraffin-embedded mouse brain sections. Antigen retrieval step: heat mediated in Citric acid pH6 buffer.Blocking step: 1%BSA for 10 mins @ rt°C. Primary antibody ab31232 incubated at 1/100 for 2 hours. Secondary antibody: anti-rabbit IgGs conjugated to biotin (1/200). The composite image shows immunostaining in cell bodies and processes of a subset of neurones in the mouse cortex (upper image) and also in the dendritic fields of the molecular layer of the mouse cerebellum (lower image). Purkinje cells of the cerebellum are negative but their primary processes are clearly delineated by a punctate membrane-region positivity. What appears to be small cells in close contact with the Purkinje cell bodies are strongly positive (perhaps these are basket cells?).

References

This product has been referenced in:
  • Chen M  et al. APP modulates KCC2 expression and function in hippocampal GABAergic inhibition. Elife 6:N/A (2017). WB ; Mouse . Read more (PubMed: 28054918) »
  • Cacciottolo M  et al. Particulate air pollutants, APOE alleles and their contributions to cognitive impairment in older women and to amyloidogenesis in experimental models. Transl Psychiatry 7:e1022 (2017). Read more (PubMed: 28140404) »

See all 35 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (adult spinal cord)
Permeabilization
Yes - Triton X-100, 0.5%
Specification
adult spinal cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 22 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
(agent) for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Commercial High pH buffer
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Sarah Franklin

Verified customer

Submitted Aug 14 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (stacking gel: 4%, resolving gel: 5-13%)
Sample
Rat Tissue lysate - whole (hippocampi total membrane fraction)
Specification
hippocampi total membrane fraction
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 02 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 10 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Cell lysate - whole cell (Mouse and Rat Hippocampus)
Specification
Mouse and Rat Hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Oct 22 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Marmoset Cerebellum)
Specification
Marmoset Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Mar 05 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Feb 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (cerebellum)
Specification
cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Feb 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted Feb 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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