Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)

Overview

  • Product nameAnti-Glutamate Receptor 1 (AMPA subtype) antibody [E308]
    See all Glutamate Receptor 1 (AMPA subtype) primary antibodies
  • Description
    Rabbit monoclonal [E308] to Glutamate Receptor 1 (AMPA subtype)
  • SpecificityThis antibody is specific for Glutamate Receptor 1 (AMPA subtype).
  • Tested applicationsSuitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminus of Human Glutamate Receptor 1 (AMPA subtype), within the first 100 amino acids.

  • Positive control
    • WB: SH-SY5Y cell lysate. IHC: human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 106 kDa (predicted molecular weight: 101 kDa).
IHC-P 1/50.
IP 1/30.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
    • Tissue specificityWidely expressed in brain.
    • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
    • Post-translational
      modifications
      Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
    • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
    • Information by UniProt
    • Database links
    • Alternative names
      • GLUR 1 antibody
      • GLUR A antibody
      • AMPA 1 antibody
      • AMPA selective glutamate receptor 1 antibody
      • AMPA-selective glutamate receptor 1 antibody
      • GluA1 antibody
      • GLUH1 antibody
      • GluR K1 antibody
      • GluR-1 antibody
      • GluR-A antibody
      • GluR-K1 antibody
      • GLUR1 antibody
      • GLURA antibody
      • GluRK1 antibody
      • Glutamate receptor 1 antibody
      • Glutamate receptor ionotropic AMPA 1 antibody
      • Glutamate receptor ionotropic antibody
      • Glutamate receptor, ionotropic, AMPA 1 antibody
      • Gria1 antibody
      • GRIA1_HUMAN antibody
      • HBGR1 antibody
      • MGC133252 antibody
      • OTTHUMP00000160643 antibody
      • OTTHUMP00000165781 antibody
      • OTTHUMP00000224241 antibody
      • OTTHUMP00000224242 antibody
      • OTTHUMP00000224243 antibody
      see all

    Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] images

    • Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436) at 1/1000 dilution + SH-SY5Y cell lysate.

      Predicted band size : 101 kDa
      Observed band size : 106 kDa (why is the actual band size different from the predicted?)
    • Ab32436, at a 1/50 dilution, staining Glutamate Receptor 1 (AMPA subtype) in paraffin embedded human brain tissue by Immunohistochemistry.
    • Immunohistochemical analysis of Human hippocampus tissue, staining Glutamate Receptor 1 (AMPA subtype) with ab32436.

      Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Samples were blocked with 10% normal goat serum for 30 minutes before incubating with primary antibody (1/20) for 2 hours. A biotinylated anti-rabbit IgG was used as the secondary antibody and staining was detected using DAB.

    References for Anti-Glutamate Receptor 1 (AMPA subtype) antibody [E308] (ab32436)

    This product has been referenced in:
    • Plaza-Zabala A  et al. An investigation of interactions between hypocretin/orexin signaling and glutamate receptor surface expression in the rat nucleus accumbens under basal conditions and after cocaine exposure. Neurosci Lett 557 Pt B:101-6 (2013). WB ; Rat . Read more (PubMed: 24262606) »
    • van Vuurden DG  et al. Attenuated AMPA receptor expression allows glioblastoma cell survival in glutamate-rich environment. PLoS One 4:e5953 (2009). IHC-P ; Human . Read more (PubMed: 19536293) »

    See all 2 Publications for this product

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