The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/2000.
By Western blot, this antibody detects a band of 100 kDa, which corresponds to the predicted molecular weight of phospho GluR 1.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
Widely expressed in brain.
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
Bonini D et al. Acute Footshock Stress Induces Time-Dependent Modifications of AMPA/NMDA Protein Expression and AMPA Phosphorylation. Neural Plast2016:7267865 (2016).
Read more (PubMed: 26966584) »
Castilho AF et al. Diabetes causes transient changes in the composition and phosphorylation of a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors and interaction with auxiliary proteins in the rat retina. Mol Vis20:894-907 (2014).
Read more (PubMed: 24966661) »