Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)

Overview

  • Product nameAnti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148]
    See all Glutamate Receptor 1 (AMPA subtype) primary antibodies
  • Description
    Rabbit monoclonal [EPR2148] to Glutamate Receptor 1 (AMPA subtype) (phospho S845)
  • Specificityab76321 only detects Glutamate Receptor 1 (AMPA subtype) phosphorylated on Serine 845.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Glutamate Receptor 1 (AMPA subtype) (phospho S845).

  • Positive control
    • WB: Human fetal, rat and mouse brain tissue lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab76321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 102 kDa.

For unpurified use at 1/1000 - 1/10000.

  • Application notesIs unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
    • Tissue specificityWidely expressed in brain.
    • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
    • Post-translational
      modifications
      Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
    • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
    • Information by UniProt
    • Database links
    • Alternative names
      • GLUR 1 antibody
      • GLUR A antibody
      • AMPA 1 antibody
      • AMPA selective glutamate receptor 1 antibody
      • AMPA-selective glutamate receptor 1 antibody
      • GluA1 antibody
      • GLUH1 antibody
      • GluR K1 antibody
      • GluR-1 antibody
      • GluR-A antibody
      • GluR-K1 antibody
      • GLUR1 antibody
      • GLURA antibody
      • GluRK1 antibody
      • Glutamate receptor 1 antibody
      • Glutamate receptor ionotropic AMPA 1 antibody
      • Glutamate receptor ionotropic antibody
      • Glutamate receptor, ionotropic, AMPA 1 antibody
      • Gria1 antibody
      • GRIA1_HUMAN antibody
      • HBGR1 antibody
      • MGC133252 antibody
      • OTTHUMP00000160643 antibody
      • OTTHUMP00000165781 antibody
      • OTTHUMP00000224241 antibody
      • OTTHUMP00000224242 antibody
      • OTTHUMP00000224243 antibody
      see all

    Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] images

    • Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1000 cells (purified) + Human fetal brain tissue lysate at 10 µg

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 102 kDa
      Observed band size : 102 kDa

      Blocking buffer: 5% NFDM/TBST.

      Diluting buffer: 1%BSA /TBST.

    • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/20000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate, untreated
      Lane 2 : Mouse brain tissue lysate, treated with Lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 102 kDa
      Observed band size : 102 kDa

      Blocking buffer: 5% NFDM/TBST.

      Diluting buffer: 1%BSA /TBST.

    • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/20000 dilution (purified)

      Lane 1 : Rat brain tissue lysate, untreated
      Lane 2 : Rat brain tissue lysate, treated with Lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 102 kDa
      Observed band size : 102 kDa

      Blocking buffer: 5% NFDM/TBST.

      Diluting buffer: 1%BSA /TBST.

    • All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/20000 dilution (unpurified)

      Lane 1 : Rat brain tissue lysate, untreated
      Lane 2 : Rat brain tissue lysate treated with Lambda Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size : 102 kDa
      Observed band size : 106 kDa (why is the actual band size different from the predicted?)

    References for Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321)

    ab76321 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76321.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"