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Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Mouse Glutamate Receptor 1 (AMPA subtype), phosphorylated at T840.
(Peptide available as ab12399.)
Our Abpromise guarantee covers the use of ab12108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 102 kDa.|
This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdomab12108 detects 2 bands over 100kDa by Western Blot. Both of these bands are blocked by the immunizing phospho peptide (lane 4), but not by the non-modified peptide (lane 5). However the immunoprecipitation data (below) demonstrates that the upper band (at ~120kDa) is unlikely to be GluR1 and therefore is an unknown protein.
This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
Lane 1 (input) has two bands >100kDa as seen in the WB for ab12108, this lane is loaded with brain extract that has not been through the IP protocol. The higher MW band in the Lane 1 (input) is probably a phospho protein as the signal is decreased when the blot is incubated with phosphatase prior to ab12108 (data not shown). However, this upper band is not GluR1 protein, as it is only detected by ab12108 and not by other GluR1 antibodies; it is therefore an unknown protein which is bound to specifically by ab12108.
Lane 3 is a control IP with mouse IgG - this is the result of using a 'pre-immune' mouse IgG antibody to see if anything is pulled down from the brain extract independent of the GluR1 antibody. The ~55kDa bands in this lane correspond to IgGs.
Lane 4 is a WB for ab12108 following IP for GluR1. This lane should only contain GluR1 (band at ~100kDa) and anything bound to it (plus IgG). The single band at the right size for GluR1 in Lane 4 is therefore likely
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