Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody (ab12108)

Overview

  • Product nameAnti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody
    See all Glutamate Receptor 1 (AMPA subtype) primary antibodies
  • Description
    Rabbit polyclonal to Glutamate Receptor 1 (AMPA subtype) (phospho T840)
  • Specificityab12108 detects two bands >100kDa in WB. The higher MW band is probably a phospho protein as the signal is decreased when the blot is incubated with phosphatase prior to ab12108 (data not shown). However, this upper band is not GluR1 protein, as it is only detected by ab12108 and not by other GluR1 antibodies; it is therefore an unknown protein which is bound to specifically by ab12108.
  • Tested applicationsSuitable for: ICC/IF, IHC-FoFr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Mouse Glutamate Receptor 1 (AMPA subtype), phosphorylated at T840.

    (Peptide available as ab12399.)

  • Positive control
    • This antibody gave a positive signal in Mouse Brain tissue lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab12108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-FoFr 1/100.
IP Use a concentration of 1 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 102 kDa.

Target

  • FunctionIonotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
  • Tissue specificityWidely expressed in brain.
  • Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily.
  • Post-translational
    modifications
    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression.
  • Information by UniProt
  • Database links
  • Alternative names
    • GLUR 1 antibody
    • GLUR A antibody
    • AMPA 1 antibody
    • AMPA selective glutamate receptor 1 antibody
    • AMPA-selective glutamate receptor 1 antibody
    • GluA1 antibody
    • GLUH1 antibody
    • GluR K1 antibody
    • GluR-1 antibody
    • GluR-A antibody
    • GluR-K1 antibody
    • GLUR1 antibody
    • GLURA antibody
    • GluRK1 antibody
    • Glutamate receptor 1 antibody
    • Glutamate receptor ionotropic AMPA 1 antibody
    • Glutamate receptor ionotropic antibody
    • Glutamate receptor, ionotropic, AMPA 1 antibody
    • Gria1 antibody
    • GRIA1_HUMAN antibody
    • HBGR1 antibody
    • MGC133252 antibody
    • OTTHUMP00000160643 antibody
    • OTTHUMP00000165781 antibody
    • OTTHUMP00000224241 antibody
    • OTTHUMP00000224242 antibody
    • OTTHUMP00000224243 antibody
    see all

Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody images

  • Lanes 1, 4, 7 & 8 : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody (ab12108) at 3 µg/ml
    Lane 2 :
    Lane 3 : Preimmune mouse IgG antibody
    Lane 5 : Blot reprobed with GluR1 mouse antibody
    Lane 6 :

    Lane 1 : Mouse brain extract that has not been through IP protocol
    Lane 2 : Blank
    Lane 3 : Control IP for mouse IgG
    Lane 4 : Mouse brain extract following IP for GluR1
    Lane 5 : As above
    Lane 6 : Blank
    Lane 7 : Input lane reprobed with GluR1 mouse antibody
    Lane 8 : IP lane reprobed with GluR1 mouse antibody


    Performed under reducing conditions.

    Predicted band size : 102 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa (possible cross reactivity),55 kDa (possible IgG).

    This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom

    Lane 1 (input) has two bands >100kDa as seen in the WB for ab12108, this lane is loaded with brain extract that has not been through the IP protocol. The higher MW band in the Lane 1 (input) is probably a phospho protein as the signal is decreased when the blot is incubated with phosphatase prior to ab12108 (data not shown). However, this upper band is not GluR1 protein, as it is only detected by ab12108 and not by other GluR1 antibodies; it is therefore an unknown protein which is bound to specifically by ab12108.

    Lane 3 is a control IP with mouse IgG - this is the result of using a 'pre-immune' mouse IgG antibody to see if anything is pulled down from the brain extract independent of the GluR1 antibody. The ~55kDa bands in this lane correspond to IgGs.

    Lane 4 is a WB for ab12108 following IP for GluR1. This lane should only contain GluR1 (band at ~100kDa) and anything bound to it (plus IgG). The single band at the right size for GluR1 in Lane 4 is therefore likely

  • Immunohistochemistical detection of Glutamate Receptor 1 (AMPA subtype) (phospho T840) using antibody ab12108 on PFA perfusion-fixed free floating rat brain sections. Primary Antibody dilution 1/100 incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). The antibody produced some staining in cytoplasmic neuronal inclusions. The picture shows the staining obtained using the objective X40 at the level of the cerebral cortex. The tissues were perfusion fixed with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.

    See Abreview

References for Anti-Glutamate Receptor 1 (AMPA subtype) (phospho T840) antibody (ab12108)

This product has been referenced in:
  • Guzman RE  et al. Involvement of ClC-3 chloride/proton exchangers in controlling glutamatergic synaptic strength in cultured hippocampal neurons. Front Cell Neurosci 8:143 (2014). WB ; Mouse . Read more (PubMed: 24904288) »
  • Gray EE  et al. Inhibitory interactions between phosphorylation sites in the C terminus of a-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-type glutamate receptor GluA1 subunits. J Biol Chem 289:14600-11 (2014). WB, IP ; Mouse . Read more (PubMed: 24706758) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted May 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (primary cortical culture)
Loading amount 10 µg
Specification primary cortical culture
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 23 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"