Anti-Glutamate Transporter 1 antibody (ab106289)

Overview

  • Product name
    Anti-Glutamate Transporter 1 antibody
  • Description
    Rabbit polyclonal to Glutamate Transporter 1
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    A 16 residue synthetic peptide derived from the C terminal domain of Rat glutamate transporter 1.

  • Positive control
    • Extracts from Rat and Mouse brains

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab106289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/200 - 1/5000. Predicted molecular weight: 62 kDa.
Flow Cyt 1/10 - 1/200. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Anti-Glutamate Transporter 1 antibody images

  • All lanes : Anti-Glutamate Transporter 1 antibody (ab106289) at 1/1000 dilution

    Lane 1 : Protein extract from Rat brain
    Lane 2 : Protein extract from Mouse brain


    Predicted band size : 62 kDa

References for Anti-Glutamate Transporter 1 antibody (ab106289)

ab106289 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (IPSC derived astrocyte)
Permeabilization
Yes - 0.1% Triton-X 100
Specification
IPSC derived astrocyte
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde
Username

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Verified customer

Submitted Mar 02 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 23 2013


Je suis ravie que vous souhaitiez participer a notre offre et tester ab106289 sur coupes de tissu humain. Le code de réduction correspondant est xx . Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre anticorps primaire....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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