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Recombinant fragment with tag: EQRDYDSRTA LHVAAAEGHV EVVKFLLEAC KVNPFPKDRW NNTPMDEALH FGHHDVFKIL QEYQVQYTPQ GDSDNGKENQ TVHKNLDGLL , corresponding to amino acids 580-670 of Human Glutaminase
Our Abpromise guarantee covers the use of ab60709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 73 kDa).|
|ELISA||Use at an assay dependent concentration. sELISA: detection limit for ab60709 is approximately 0.3ng/ml as a capture antibody.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|IP||Use at an assay dependent concentration.|