• Product nameAnti-Glutaminase antibody
    See all Glutaminase primary antibodies
  • Description
    Rabbit polyclonal to Glutaminase
  • Tested applicationsSuitable for: Flow Cyt, WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Glutaminase aa 516-545 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • WB: 293, 239T and HeLa cell lysates, mouse liver and brain tissue lysates, human brain and rat kidney tissue lysates. ICC/IF: HeLa and HepG2 cells. IHC-P: Human liver and mouse brain tissues. Flow Cyt: HepG2 cells.



Our Abpromise guarantee covers the use of ab93434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Predicted molecular weight: 65 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • FunctionCatalyzes the first reaction in the primary pathway for the renal catabolism of glutamine.
  • Tissue specificityKGA is expressed predominantly in brain and kidney but not in liver, isoform 3 is expressed principally in cardiac muscle and pancreas but not in liver or brain, and isoform 2 is expressed solely in cardiac and skeletal muscle.
  • Sequence similaritiesBelongs to the glutaminase family.
    Contains 1 ANK repeat.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAD20 antibody
    • DKFZp686O15119 antibody
    • FLJ10358 antibody
    • GAC antibody
    • GAM antibody
    • GLS antibody
    • GLS1 antibody
    • GLSK_HUMAN antibody
    • Glutaminase C antibody
    • Glutaminase kidney isoform antibody
    • Glutaminase phosphate activated antibody
    • K-glutaminase antibody
    • KGA antibody
    • KIAA0838 antibody
    • L-glutamine amidohydrolase antibody
    • mitochondrial antibody
    see all

Anti-Glutaminase antibody images

  • All lanes : Anti-Glutaminase antibody (ab93434) at 1/1000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Human brain tissue lysate
    Lane 5 : Mouse brain tissue lysate

    Lysates/proteins at 35 µg per lane.

    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 65 kDa
  • Anti-Glutaminase antibody (ab93434) at 1/1000 dilution + Rat kidney tissue lyate at 35 µg

    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 65 kDa
  • Anti-Glutaminase antibody (ab93434) at 1/1000 dilution + mouse liver tissue lysate at 35 µg

    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size : 65 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • ICC/IF image of ab93434 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93434, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Glutaminase (green) with ab93434. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Counter stained with DAPI (blue).

  • IHC image of ab93434 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93434, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue labelling Glutaminase with ab93434. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody.

  • Flow cytometry analysis of HepG2 cells labelling Glutaminase (green) with ab93434 compared to a negative control (blue). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.

References for Anti-Glutaminase antibody (ab93434)

This product has been referenced in:
  • Huang F  et al. Expression of glutaminase is upregulated in colorectal cancer and of clinical significance. Int J Clin Exp Pathol 7:1093-100 (2014). WB, IHC-P ; Human . Read more (PubMed: 24696726) »
  • Arroyo JD  et al. Systematic design and functional analysis of artificial microRNAs. Nucleic Acids Res 42:6064-77 (2014). WB ; Human . Read more (PubMed: 24598260) »

See all 3 Publications for this product

Product Wall

Thank you for your email.

Unfortunately, the exact sequence of the immunogen for ab93434 is confidential and I was denied access to it.

But I am happy to confirm that ab93434 is generated from rabbits immunized with a KLH conjugated...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain section - cortical neurons)
Specification Brain section - cortical neurons
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C

Dr. Richard Fairless

Verified customer

Submitted Mar 18 2013

Expiration date: 18. March 2013
Value: €370.00

I am very pleased to hear you would like to accept our offer and test ab93434 in rat. This code will give you €370.00 off your next order before th...

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Thank you very much for your interest our products.

I am happy to confirm that the immunogen of ab93434 shares a 90% homology with rat and therefore it is likely that ab93434 cross reacts with rat.

To our knowledge, ab93434 ha...

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Thank you for contacting us.

The antibody is raised against an amino acid sequence derived from the kidney isoform, product of the GLS gene, but the sequence shares 87% identity with the product of the GLS2 gene, the liver isoform. This prob...

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Thank you for your inquiry.

This antibody is for Glutaminase kidney isoform, mitochondrial. The antibody will recognize isoforms 1 & 3.


Out tested positive control in WB is mouse liver...

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Thank you for your enquiry.

I am sorry to confirm that ab93434 and ab60709 have not been specifically tested on brain tissue in western blot and so regrettably there is no data to provide.
I would like to reassure you that ab93434 and a...

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Thank you for providing me with this extra information. It has allowed me to more fully understand the problems you have been experiencing with ab49873 and ab93434 previously.

Having looked at the protocol I have a few further questions:
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Thank you for your reply.

I am very happy to hear that the protocol tips improve the result.

Aspartate Aminotransferase has three known phosphorylation sites. Phosphorylation has an effect on the molecular weighta protein shows on a...

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Expiration date: 14 June 2012

I am very pleased to hear you would like to accept our offer and test ab93434 in RAT-WB. This code will give you 1 free [PRIMARY ANTIBOD] before the expiration date. To redeem this off...

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