Glutamine Assay Kit (Colorimetric) (ab197011)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 90 min
- Sample type: Other biological fluids, Plasma, Serum, Tissue, Urine
- Sensitivity: 25 µM
Overview
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Product name
Glutamine Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue -
Assay type
Quantitative -
Sensitivity
25 µM -
Assay time
0h 90m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Glutamine Assay kit (Colorimetric) ab197011 is a simple and sensitive assay that detects the biologically relevant concentrations of glutamine in biological fluids and tissues.
The glutamine assay protocol is based on the hydrolysis of Glutamine to Glutamate producing a stable colored signal, which is directly proportional to the amount of glutamine in the sample.
The assay can detect as little as 25 μM of glutamine in a variety of biological samples.
Glutamine assay protocol summary:
- add samples and standards to wells
- add hydrolysis mix and incubate for 30 min
- add reaction mix and incubate for 60 min
- analyze with a microplate reader. -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K556 Glutamine Colorimetric Assay Kit. K556-100 is the same size as the 100 test size of ab197011.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer XXIX 1 x 25ml Assay Buffer XXXI 1 x 25ml Developer Solution III 1 vial Enzyme Mix VIII 1 vial Glutamine Standard 1 vial Hydrolysis Enzyme Mix II 1 vial -
Research areas
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Relevance
Glutamine (abbreviated as Gln or Q; the abbreviation Glx or Z represents either glutamine or glutamic acid) is one of the 20 amino acids encoded by the standard genetic code. Its side chain is an amide formed by replacing the side-chain hydroxyl of glutamic acid with an amine functional group. It can therefore be considered the amide of glutamic acid. Its codons are CAA and CAG. Recent studies have proven its effectiveness in anabolic muscle growth from prolonged consumption. -
Alternative names
- Gln
- Q
Images
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Glutamine Standard Curve. For demonstrative purposes only.
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Measurement of Glutamine concentration in Human urine (5 μl).
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Measurement of Glutamine concentration in Mouse tissues (brain [15 μg], liver [50 μg]). Samples were deproteinized using 10K Spin Column and spiked with known amount of Glutamine (6 nmol).
Datasheets and documents
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SDS download
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Datasheet download
References (31)
ab197011 has been referenced in 31 publications.
- Zhang K et al. M2 macrophage-derived exosomal miR-193b-3p promotes progression and glutamine uptake of pancreatic cancer by targeting TRIM62. Biol Direct 18:1 (2023). PubMed: 36631876
- Ge J et al. Inhibition of lncRNA NEAT1 sensitizes medulloblastoma cells to cisplatin through modulating the miR-23a-3p-glutaminase (GLS) axis. Bioengineered 13:7670-7682 (2022). PubMed: 35313796
- Tang Y et al. Simultaneous glutamine metabolism and PD-L1 inhibition to enhance suppression of triple-negative breast cancer. J Nanobiotechnology 20:216 (2022). PubMed: 35524267
- Wu B et al. ADSCs-derived exosomes ameliorate hepatic fibrosis by suppressing stellate cell activation and remodeling hepatocellular glutamine synthetase-mediated glutamine and ammonia homeostasis. Stem Cell Res Ther 13:494 (2022). PubMed: 36195966
- Feng Y et al. Pharmacological Inhibition of Glutaminase 1 Attenuates Alkali-Induced Corneal Neovascularization by Modulating Macrophages. Oxid Med Cell Longev 2022:1106313 (2022). PubMed: 35345831