Anti-Glutamine Synthetase antibody [6/Glutamine Synthetase] (ab125724)
Key features and details
- Mouse monoclonal [6/Glutamine Synthetase] to Glutamine Synthetase
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Glutamine Synthetase antibody [6/Glutamine Synthetase]
See all Glutamine Synthetase primary antibodies -
Description
Mouse monoclonal [6/Glutamine Synthetase] to Glutamine Synthetase -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep -
Immunogen
Full length protein, corresponding to amino acids 1-373 of Sheep Glutamine Synthetase.
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Positive control
- Human liver tissue; Human Hepatocellular carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Buffer with protein carrier and preservative. -
Concentration information loading...
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Purification notes
Purity >90% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
6/Glutamine Synthetase -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/150 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/150 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts. -
Involvement in disease
Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. -
Sequence similarities
Belongs to the glutamine synthetase family. -
Developmental stage
Expressed during early fetal stages. -
Cellular localization
Cytoplasm. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 2752 Human
- Omim: 138290 Human
- SwissProt: P15104 Human
- Unigene: 518525 Human
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Alternative names
- cell proliferation-inducing protein 59 antibody
- Cgl2214 antibody
- GLNA antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab125724 has been referenced in 5 publications.
- Todd L et al. Retinoic Acid-Signaling Regulates the Proliferative and Neurogenic Capacity of Müller Glia-Derived Progenitor Cells in the Avian Retina. Stem Cells 36:392-405 (2018). PubMed: 29193451
- Todd L et al. BMP- and TGFß-signaling regulate the formation of Müller glia-derived progenitor cells in the avian retina. Glia 65:1640-1655 (2017). PubMed: 28703293
- Todd L et al. Comparative analysis of glucagonergic cells, glia, and the circumferential marginal zone in the reptilian retina. J Comp Neurol 524:74-89 (2016). PubMed: 26053997
- Todd L et al. Jak/Stat signaling regulates the proliferation and neurogenic potential of Müller glia-derived progenitor cells in the avian retina. Sci Rep 6:35703 (2016). PubMed: 27759082
- Todd L et al. Heparin-binding EGF-like growth factor (HB-EGF) stimulates the proliferation of Müller glia-derived progenitor cells in avian and murine retinas. Mol Cell Neurosci 69:54-64 (2015). PubMed: 26500021