Overview

  • Product nameAnti-Glutamine Synthetase antibody
    See all Glutamine Synthetase primary antibodies
  • Description
    Rabbit polyclonal to Glutamine Synthetase
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cow, Dog, Chimpanzee, Ferret, Zebrafish
  • Immunogen

    Recombinant full length protein purified from E.coli (Human).

  • Positive control
    • Mouse and Rat brain extract. Mouse liver extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab16802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 42 kDa.
IHC-FoFr 1/100.

Target

  • FunctionThis enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
  • Involvement in diseaseDefects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
  • Sequence similaritiesBelongs to the glutamine synthetase family.
  • Developmental stageExpressed during early fetal stages.
  • Cellular localizationCytoplasm. Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • cell proliferation-inducing protein 59 antibody
    • GLNA antibody
    • GLNA_HUMAN antibody
    • GLNS antibody
    • GLUL antibody
    • Glutamate ammonia ligase antibody
    • Glutamate decarboxylase antibody
    • Glutamate--ammonia ligase antibody
    • glutamine synthase antibody
    • Glutamine synthetase antibody
    • GS antibody
    • PIG 43 antibody
    • PIG 59 antibody
    • PIG43 antibody
    • PIG59 antibody
    • Proliferation inducing protein 43 antibody
    see all

Anti-Glutamine Synthetase antibody images

  • Left panel shows scanned image of an amine coated plate seeded with a titration of HeLa cells and stained with different dilutions of ab16082 using In Cell ELISA support pack ab111542 and protocol. Right panel shows bar graph of normalized signal intensity in 3 different cells lines after staining with ab160802 at 1/2500 dilution. Average intra-assay coefficient of variation was calculated at 5.6% and Z score at 0.77.

  • HeLa cells were seeded on glass coverslips and allow to adhere overnight. Cells were fixed with 4% paraformaldehyde for 15 min and washed in PBS x3. ICC procedure was carried out using In Cell ELISA support pack ab111541 and protocol. Left panel shows HeLa cells stained with ab16802 at a dilution of 1/1000, labeled with Goat anti-rabbit 594 secondary antibody and counterstain with DAPI. Right panel shows co-localization of the signal with the mitochondrial marker Anti-ATPB antibody [3D5] ab14730 (4 �g/mL) labeled with Goat anti-mouse 488 secondary antibody.


  • Predicted band size : 42 kDa
    ab16802 at a 1/2000 dilution staining approx. 42kDa Glutamine synthetase in 1) 293T cell lysate transfected with Myc-Glutamine synthetase, 2) mouse brain lysate, 3) mouse liver lysate and 4) rat brain lysate by Western blot (ECL). ab16802 at a 1/2000 dilution staining approx. 42kDa Glutamine synthetase in 1) 293T cell lysate transfected with Myc-Glutamine synthetase, 2) mouse brain lysate, 3) mouse liver lysate and 4) rat brain lysate by Western blot (ECL).
  • ICC/IF image of ab16802 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16802, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Glutamine Synthetase antibody (ab16802)

This product has been referenced in:
  • Barcelona PF  et al. p75NTR and Its Ligand ProNGF Activate Paracrine Mechanisms Etiological to the Vascular, Inflammatory, and Neurodegenerative Pathologies of Diabetic Retinopathy. J Neurosci 36:8826-41 (2016). Read more (PubMed: 27559166) »
  • Burkovetskaya M  et al. Evidence for Aberrant Astrocyte Hemichannel Activity in Juvenile Neuronal Ceroid Lipofuscinosis (JNCL). PLoS One 9:e95023 (2014). IHC-Fr, WB ; Mouse, Human . Read more (PubMed: 24736558) »

See all 11 Publications for this product

Product Wall

Thank you for contacting us.
I am sorry that the antibody is not working as expected.
If you please could send me an image of your staining, that would be of great help.
I'd be happy to further discuss options of resolving this case. Read More

Thank you for contacting us.

We carry 13 antibodies to glutamine synthetase. None of these have so far been tested in yeast.

I have 2 questions for you:

1) What application/technique are you planning to use the antibody i...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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Thank you for your reply.
I think you would not really see an overlap with the nucleus as GS is more located in the cytoplasm and mitochondria, but the staining should be more intense in general.
I'd be happy to replace, credit or refund the ...

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Thank you for your email and the image.
The staining does look like non-specific staining. I have a couple questions in this regard:
1) What antibody dilution was used for this image?
2) Where would the nuclei be located in relation to t...

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Thank you for your enquiry. We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instr...

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