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Our Abpromise guarantee covers the use of ab49873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000. Predicted molecular weight: 42 kDa.|
|IHC-P||1/10000 - 1/20000.|
|ICC/IF||Use at an assay dependent concentration.|
ab49873 at 1/5000 staining cultured mouse astroglial cells by ICC/IF. The cells were paraformaldehyde fixed and permeabilized with TritonX100 before being blocked and stained with the antibody for 24 hours at 4°C. An Alexa-Fluor ® 594 conjugated chicken anti-rabbit antibody was used as the secondary.