Overview

  • Product name
    Anti-Glutamine Synthetase antibody
    See all Glutamine Synthetase primary antibodies
  • Description
    Rabbit polyclonal to Glutamine Synthetase
  • Specificity
    ab49873 recognizes glutamine synthetase (GS) (45 kDA).
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Monkey
    Predicted to work with: Chicken, Hamster, Cow, Dog, Human, Pig, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    RTCLLNETGDEPFQYKN

    , corresponding to C terminal amino acids 357-373 of Mouse Glutamine Synthetase

  • Positive control
    • Rat brain tissue, rat brain cytosolic fraction extract, cerebellum, kidney

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Ion Exchange Chromatography
  • Purification notes
    Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 42 kDa.
IHC-P 1/10000 - 1/20000.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/5000.

Target

  • Function
    This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
  • Involvement in disease
    Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
  • Sequence similarities
    Belongs to the glutamine synthetase family.
  • Developmental stage
    Expressed during early fetal stages.
  • Cellular localization
    Cytoplasm. Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • cell proliferation-inducing protein 59 antibody
    • GLNA antibody
    • GLNA_HUMAN antibody
    • GLNS antibody
    • GLUL antibody
    • Glutamate ammonia ligase antibody
    • Glutamate decarboxylase antibody
    • Glutamate--ammonia ligase antibody
    • glutamine synthase antibody
    • Glutamine synthetase antibody
    • GS antibody
    • PIG 43 antibody
    • PIG 59 antibody
    • PIG43 antibody
    • PIG59 antibody
    • Proliferation inducing protein 43 antibody
    see all

Images

  • Lane 1 :
    Lane 2 :
    Lane 3 :

    Lane 1 : As above
    Lane 2 : 10000
    Lane 3 : 20000

    Secondary
    Goat Anti-Rabbit IgG-Peroxidase

    Predicted band size : 42 kDa
  • ab49873 at 1/5000 staining cultured mouse astroglial cells by ICC/IF. The cells were paraformaldehyde fixed and permeabilized with TritonX100 before being blocked and stained with the antibody for 24 hours at 4°C. An Alexa-Fluor ® 594 conjugated chicken anti-rabbit antibody was used as the secondary.

    See Abreview

  • Glutamine Synthetase antibody (ab49873) immunohistochemistry on rat brain and kidney tissue (formalin/PFA-fixed paraffin-embedded) sections. Rat cerebellum and rat kidney sections were incubated with ab49873 (1/21000) for 2h at RT. Antigen retrieval was performed by heat induction in citrate buffer pH6. The upper panel shows immunoreactivity in rat kidney, apart from convoluted tubule positivity (proximal: see asterisks) there is positivity within the glomerular capsule (perhaps the parietal epithelium: see arrows). In the lower image (cerebellum), apart from astrocytic glial cell body and process positivity, I note that what I think are oligodendrocytes in the grey matter, are also positive. In the corpus callosum of the same section (and all other major axonal tracks of this brain), linearly arranged cells are also positive: these are known to be oligodendrocytes.
    .

    See Abreview

References

This product has been referenced in:
  • Zhou M  et al. Non-cell-autonomous activation of IL-6/STAT3 signaling mediates FGF19-driven hepatocarcinogenesis. Nat Commun 8:15433 (2017). IHC-P ; Mouse . Read more (PubMed: 28508871) »
  • Valbuena GN  et al. Metabolomic Analysis Reveals Increased Aerobic Glycolysis and Amino Acid Deficit in a Cellular Model of Amyotrophic Lateral Sclerosis. Mol Neurobiol 53:2222-40 (2016). Read more (PubMed: 25963727) »

See all 18 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1 Ventana (high pH)
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Dr. Enrico Radaelli

Verified customer

Submitted Aug 21 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Jugular nodose ganglion complex (JNC))
Antigen retrieval step
None
Permeabilization
No
Specification
Jugular nodose ganglion complex (JNC)
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5 · Temperature: RT°C
Fixative
Zamboni
Username

Mr. Duc Dung Le

Verified customer

Submitted Apr 20 2015

Application
IHC - Wholemount
Sample
Mouse Tissue (Retina)
Specification
Retina
Username

Abcam user community

Verified customer

Submitted Feb 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Mar 12 2013

Thank you for your call today and for letting us know about this issue.
I've created a discount code that can be used to receive 20% off a vial of ab49873. Please include the code below with your order to redeem this offer:
Discount Code: ***...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Wie am Telefon diskutiert, ist unseres Wissens nach wurde der ab49873 bisher noch nicht inauf humanem Gewebegetestet. Falls Sie dies selbst testen möchten...

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That's no problem. I am sorry for the confusion, I should have made it clear to you that they would be arriving in the same box.

If I can help in any other way please do let me know. Otherwise, I wish you all the best with your research.

That is no problem. If you have any problems in the future, please do not hesitate to contact us again.

Until then, I wish you all the best with your research.

Thank you for getting back to me.

I am sorry for the frustration that this situation has caused you. I will arrange for ab91073 to be sent out to you as discussed. I can also arrange for ab60709 to be sent as a replacement for ab49873 which y...

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Thank you for getting back to me.

I am sorry that the reply to your fax was sent to your supervisor by one of my colleagues, Keith. This was due to the way our assigning system works. The protocol tips that I gave and those of Keith are not t...

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