Anti-Glutamine Synthetase antibody (ab49873)


  • Product nameAnti-Glutamine Synthetase antibody
    See all Glutamine Synthetase primary antibodies
  • Description
    Rabbit polyclonal to Glutamine Synthetase
  • Specificityab49873 recognizes glutamine synthetase (GS) (45 kDA).
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Monkey
    Predicted to work with: Chicken, Hamster, Cow, Dog, Human, Pig, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:


    , corresponding to C terminal amino acids 357-373 of Mouse Glutamine Synthetase

  • Positive control
    • Rat brain tissue, rat brain cytosolic fraction extract, cerebellum, kidney


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityIon Exchange Chromatography
  • Purification notesWhole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab49873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 42 kDa.
IHC-P 1/10000 - 1/20000.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/5000.


  • FunctionThis enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
  • Involvement in diseaseDefects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
  • Sequence similaritiesBelongs to the glutamine synthetase family.
  • Developmental stageExpressed during early fetal stages.
  • Cellular localizationCytoplasm. Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • cell proliferation-inducing protein 59 antibody
    • GLNA antibody
    • GLNA_HUMAN antibody
    • GLNS antibody
    • GLUL antibody
    • Glutamate ammonia ligase antibody
    • Glutamate decarboxylase antibody
    • Glutamate--ammonia ligase antibody
    • glutamine synthase antibody
    • Glutamine synthetase antibody
    • GS antibody
    • PIG 43 antibody
    • PIG 59 antibody
    • PIG43 antibody
    • PIG59 antibody
    • Proliferation inducing protein 43 antibody
    see all

Anti-Glutamine Synthetase antibody images

  • ab49873 at 1/5000 staining cultured mouse astroglial cells by ICC/IF. The cells were paraformaldehyde fixed and permeabilized with TritonX100 before being blocked and stained with the antibody for 24 hours at 4°C. An Alexa-Fluor ® 594 conjugated chicken anti-rabbit antibody was used as the secondary.

    See Abreview

  • Glutamine Synthetase antibody (ab49873) immunohistochemistry on rat brain and kidney tissue (formalin/PFA-fixed paraffin-embedded) sections. Rat cerebellum and rat kidney sections were incubated with ab49873 (1/21000) for 2h at RT. Antigen retrieval was performed by heat induction in citrate buffer pH6. The upper panel shows immunoreactivity in rat kidney, apart from convoluted tubule positivity (proximal: see asterisks) there is positivity within the glomerular capsule (perhaps the parietal epithelium: see arrows). In the lower image (cerebellum), apart from astrocytic glial cell body and process positivity, I note that what I think are oligodendrocytes in the grey matter, are also positive. In the corpus callosum of the same section (and all other major axonal tracks of this brain), linearly arranged cells are also positive: these are known to be oligodendrocytes.

    See Abreview

References for Anti-Glutamine Synthetase antibody (ab49873)

This product has been referenced in:
  • Eakins R  et al. Adaptation to acetaminophen exposure elicits major changes in expression and distribution of the hepatic proteome. Sci Rep 5:16423 (2015). Read more (PubMed: 26607827) »
  • Zhang S  et al. TALEN-mediated somatic mutagenesis in murine models of cancer. Cancer Res 74:5311-21 (2014). Mouse . Read more (PubMed: 25070752) »

See all 11 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Jugular nodose ganglion complex (JNC))
Antigen retrieval step None
Permeabilization No
Specification Jugular nodose ganglion complex (JNC)
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5 · Temperature: RT°C
Fixative Zamboni

Mr. Duc Dung Le

Verified customer

Submitted Apr 20 2015

Application IHC - Wholemount
Sample Mouse Tissue (Retina)
Specification Retina

Abcam user community

Verified customer

Submitted Feb 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Mar 12 2013

Thank you for your call today and for letting us know about this issue.
I've created a discount code that can be used to receive 20% off a vial of ab49873. Please include the code below with your order to redeem this offer:
Discount Code: ***...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Wie am Telefon diskutiert, ist unseres Wissens nach wurde der ab49873 bisher noch nicht inauf humanem Gewebegetestet. Falls Sie dies selbst testen möchten...

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That's no problem. I am sorry for the confusion, I should have made it clear to you that they would be arriving in the same box.

If I can help in any other way please do let me know. Otherwise, I wish you all the best with your research.

That is no problem. If you have any problems in the future, please do not hesitate to contact us again.

Until then, I wish you all the best with your research.

Thank you for getting back to me.

I am sorry for the frustration that this situation has caused you. I will arrange for ab91073 to be sent out to you as discussed. I can also arrange for ab60709 to be sent as a replacement for ab49873 which y...

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Thank you for getting back to me.

I am sorry that the reply to your fax was sent to your supervisor by one of my colleagues, Keith. This was due to the way our assigning system works. The protocol tips that I gave and those of Keith are not t...

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Thank you for providing me with this extra information. It has allowed me to more fully understand the problems you have been experiencing with ab49873 and ab93434 previously.

Having looked at the protocol I have a few further questions:
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