• Product nameAnti-Glutamine Synthetase antibody
    See all Glutamine Synthetase primary antibodies
  • Description
    Goat polyclonal to Glutamine Synthetase
  • Tested applicationsSuitable for: WB, IP, ELISA, Dot Blotmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Glutamine Synthetase [Microbial]


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
  • PurityWhole antiserum
  • Purification notesThis product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutamine Synthetase [Microbial].
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab6585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot Blot
  • Application notesimmunoblotting (western or dot blot), ELISA (1:10,000 to 1:40,000), immunoprecipitation and most immunological methods requiring high titer and specificity.
  • Target

    • FunctionThis enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
    • Involvement in diseaseDefects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD) [MIM:610015]. CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid.
    • Sequence similaritiesBelongs to the glutamine synthetase family.
    • Developmental stageExpressed during early fetal stages.
    • Cellular localizationCytoplasm. Mitochondrion.
    • Information by UniProt
    • Alternative names
      • cell proliferation-inducing protein 59 antibody
      • GLNA antibody
      • GLNA_HUMAN antibody
      • GLNS antibody
      • GLUL antibody
      • Glutamate ammonia ligase antibody
      • Glutamate decarboxylase antibody
      • Glutamate--ammonia ligase antibody
      • glutamine synthase antibody
      • Glutamine synthetase antibody
      • GS antibody
      • PIG 43 antibody
      • PIG 59 antibody
      • PIG43 antibody
      • PIG59 antibody
      • Proliferation inducing protein 43 antibody
      see all

    References for Anti-Glutamine Synthetase antibody (ab6585)

    This product has been referenced in:
    • Ivanovska N & Dimitrova P Bone resorption and remodeling in murine collagenase-induced osteoarthritis after administration of glucosamine. Arthritis Res Ther 13:R44 (2011). IHC-P ; Mouse . Read more (PubMed: 21410959) »
    • Yuan J  et al. Metabolomics-driven quantitative analysis of ammonia assimilation in E. coli. Mol Syst Biol 5:302 (2009). WB ; Escherichia coli . Read more (PubMed: 19690571) »

    See all 2 Publications for this product

    Product Wall

    They have informed me that for both ab6585 and ab34525, they do not have any data on possible cross-reactivity with Streptomyces lincolnensis. The actual immunogen is not sequenced but a reference sequence for comparison is shown at: http://www.un...

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    I suggest that you conduct your search as normal through the Abcam Website. As you cannot find a product within the Abcam catalog that meets your needs, click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search ...

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    I've checked our previous enquiries for this antibody and it seems that there are lots of questions regarding the nature of the immunogen, but I'm afraid that I do not know from which microbe the GS was derived. The antibody has not specifically been t...

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    We have contacted the manufacturer of the enzyme used, and they can't tell us which isoform of Gln synthetase was used. Apparently, they measure activity in a set assay and if the enzyme (or enzymes) perform properly, they don't worry about which isofo...

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    This antibody was raised against a microbial glutamine synthetase and has not been specifically tested for cross-reactivity with the rat enzyme. Neither has it been used previously in immunocytochemistry, however it is thought likely that it would work...

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    This antibody has been raised against microbial glutamine synthetase and does not react with the mouse protein.

    This antibody has not been specifically tested in IHC and IF. Unfortunately, we do not know of any references where this antibody has been used.